CC BY 4.0 · Glob Med Genet 2023; 10(03): 159-163
DOI: 10.1055/s-0043-1770957
Original Research

IL-10 Polymorphism and Breast Cancer Risk in Georgian Women: A Case–Control Study

Saba Ahmadi
1   Department of Molecular and Medical Genetics, Tbilisi State Medical University, Tbilisi, Georgia
Sandro Surmava
1   Department of Molecular and Medical Genetics, Tbilisi State Medical University, Tbilisi, Georgia
Eka Kvaratskhelia
1   Department of Molecular and Medical Genetics, Tbilisi State Medical University, Tbilisi, Georgia
Elene Abzianidze
1   Department of Molecular and Medical Genetics, Tbilisi State Medical University, Tbilisi, Georgia
Ketevani Kankava
1   Department of Molecular and Medical Genetics, Tbilisi State Medical University, Tbilisi, Georgia
› Author Affiliations
Funding This study was supported by the Shota Rustaveli National Science Foundation of Georgia (grant number #FR-21-17599).


Background Interleukin-10 (IL-10) is a cytokine with a vast variety of functions, but its role in cancer development and progression is not yet clear. It is involved in two of the hallmarks of cancer: vascularization and immune modulation. IL-10 inhibits angiogenesis and hence is antitumorigenic. But it also can suppress the immune system and be tumorigenic.

Objective Evaluating the role of IL-10 (-1082 A/G) gene promoter single-nucleotide polymorphism (SNP) in breast cancer susceptibility and progression in Georgian women.

Methods A case–control study was performed on a total of 128 women, with 64 of them being histologically confirmed to have breast cancer and 64 healthy controls. SNP genotyping was performed with TaqMan assay with real-time polymerase chain reaction. And pathology report, containing proliferative activity and breast cancer hormonal status, was obtained after surgery of the case individuals. Statistical analysis was done to investigate the significance of data obtained from genotyping and histology reports.

Results Statistical analysis revealed that the difference in frequency of genotypes was not statistically significant between cases and controls (chi-square = 0.5812, p = 0.7478). The comparison of proliferative activity of cases with AA genotypes and AG/GG genotypes showed no statistical difference (t = 0.2575, p = 0.7980). Although when put into a plot (box and whiskers), patients with AG/GG genotype have outliers with very high proliferative activity.

Conclusion This study shows that -1082 A/G SNP in the promoter region of the IL-10 gene is not associated with breast cancer risk in Georgian women.

Ethics Approval

The study is approved by the Ethics Committee of Tbilisi State Medical University, Georgia under approval number #44/3 with the following members: 1. Givi Javashvili, Chair of the Ethics Committee; 2. Guram Kiknadze; 3. Tamar Kurtanidze; 4. Davit Gelovani; 5. Irma Manjavidze; 6. Ramaz Shengelia; 7. Temur Silagadze; 8. Zaza Chafichadze; 9. Zaza Berishvili; 10. Magda Bethaneli; 11. Akaki Barkalaya; 12. Gocha Shatirishvili.

Consent to Participate

Signed informed consent was collected from every study participant.

Availability of Data and Materials

In case you want to access our PCR raw data, you can contact the first author by email at:

Publication History

Article published online:
10 July 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (

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