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CC BY 4.0 · Eur J Dent 2024; 18(01): 397-400
DOI: 10.1055/s-0043-1769900
Brief Report

Effect of Chlorhexidine and Tea Tree Oil on Reducing the Number of Oral Microorganisms

André Luiz de Melo Moreno
1   Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil
,
Clóvis Lamartine de Moraes Melo Neto 
1   Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil
,
Marcelo Coelho Goiato 
1   Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil
2   Oral Oncology Center, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil
,
Nathaly Vilene de Araujo Moreno
3   Courses for Dentistry (Instituto de Excelência em Ensino e Pesquisa), Manaus, Amazonas, Brazil
,
Daniela Micheline dos Santos
1   Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil
2   Oral Oncology Center, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil
,
Cássia Cunha de Lima
1   Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil
,
Rogério Heládio Lopes Motta
4   Area of Pharmacology, Anesthesiology and Therapeutics, São Leopoldo Mandic University, School of Dentistry, Campinas, São Paulo, Brazil
,
Juliana Cama Ramacciato 
4   Area of Pharmacology, Anesthesiology and Therapeutics, São Leopoldo Mandic University, School of Dentistry, Campinas, São Paulo, Brazil
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Abstract

Objectives Thus, the aim of this study was to compare the effect of using two preoperative mouthwashes (0.12% chlorhexidine and 0.2% tea tree oil) on the number of colonies of oral microorganisms.

Materials and Methods Forty participants who needed to be rehabilitated with dental implants were included in this study. They were randomly divided into two groups (chlorhexidine group and tea tree group; n = 20, each). For each group, saliva samples were collected at four different times: T0 (initially)—before using the mouthwash, T1—after 1 minute of using the mouthwash, T10—after 10 minutes of using the mouthwash, and T60—after 60 minutes of using the mouthwash. At T0 and T1, saliva samples were collected before implant placement surgery, and at T10 and T60, saliva samples were collected during surgery. In each group, one saliva sample was collected at each evaluated time point for each patient, totaling 4 saliva collections per patient. MSB agar (Mitis-Salivarius-Bacitracin) and BHI agar (Brain Heart Infusion) culture media were used in each group. Microbial colony counts were performed using a magnifying glass and recorded in CFU (colony forming units)/mL. Statistical analyses were performed using the Friedman, Mann–Whitney U and Wilcoxon tests (p < 0.05).

Results Based on MSB agar culture medium, at T0, the number of Streptococcus mutans colonies in the chlorhexidine group was significantly higher compared with the tea tree group (p <0.05; MSB agar). The chlorhexidine group showed significantly lower CFU/mL values for Streptococcus mutans at T1, T10, and T60 compared with the tea tree group (p <0.05; MSB agar). Based on BHI agar culture medium, at T0, the chlorhexidine group showed a significantly lower value of CFU/mL compared with the tea tree group (p < 0.05; BHI agar). At T1, T10, and T60, the chlorhexidine group showed significantly lower CFU/mL values compared with the tea tree group (p <0.05; BHI agar).

Conclusion Chlorhexidine is more indicated as a preoperative mouthwash than tea tree oil, due to its significantly more effective antimicrobial action.

Keywords

chlorhexidine - tea tree oil - dental plaque - dental implants - mouthwashes - Streptococcus mutans - Melaleuca


Publication History

Article published online:
02 August 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Dhaliwal JS, Abd Rahman NA, Ming LC, Dhaliwal SKS, Knights J, Albuquerque Junior RF. Microbial biofilm decontamination on dental implant surfaces: a mini review. Front Cell Infect Microbiol 2021; 11: 736186
    CrossrefPubMedSearch in Google Scholar
  • 2 Solderer A, Kaufmann M, Hofer D, Wiedemeier D, Attin T, Schmidlin PR. Efficacy of chlorhexidine rinses after periodontal or implant surgery: a systematic review. Clin Oral Investig 2019; 23 (01) 21-32
    CrossrefPubMedSearch in Google Scholar
  • 3 Lam NS, Long X, Su XZ, Lu F. Melaleuca alternifolia (tea tree) oil and its monoterpene constituents in treating protozoan and helminthic infections. Biomed Pharmacother 2020; 130: 110624
    CrossrefPubMedSearch in Google Scholar
  • 4 Carson CF, Hammer KA, Riley TV. Melaleuca alternifolia (tea tree) oil: a review of antimicrobial and other medicinal properties. Clin Microbiol Rev 2006; 19 (01) 50-62
    CrossrefPubMedSearch in Google Scholar
  • 5 Taalab MR, Mahmoud SA, Moslemany RME, Abdelaziz DM. Intrapocket application of tea tree oil gel in the treatment of stage 2 periodontitis. BMC Oral Health 2021; 21 (01) 239
    CrossrefPubMedSearch in Google Scholar
  • 6 Santamaria Jr M, Petermann KD, Vedovello SA, Degan V, Lucato A, Franzini CM. Antimicrobial effect of Melaleuca alternifolia dental gel in orthodontic patients. Am J Orthod Dentofacial Orthop 2014; 145 (02) 198-202
    CrossrefPubMedSearch in Google Scholar
  • 7 Ripari F, Cera A, Freda M, Zumbo G, Zara F, Vozza I. Tea tree oil versus chlorhexidine mouthwash in treatment of gingivitis: a pilot randomized, double blinded clinical trial. Eur J Dent 2020; 14 (01) 55-62
    Thieme ConnectPubMedSearch in Google Scholar
  • 8 Groppo FC, Ramacciato JC, Simões RP, Flório FM, Sartoratto A. Antimicrobial activity of garlic, tea tree oil, and chlorhexidine against oral microorganisms. Int Dent J 2002; 52 (06) 433-437
    CrossrefPubMedSearch in Google Scholar
  • 9 Bisht A, Hemrajani C, Upadhyay N. et al. Azelaic acid and Melaleuca alternifolia essential oil co-loaded vesicular carrier for combinational therapy of acne. Ther Deliv 2022; 13 (01) 13-29
    CrossrefPubMedSearch in Google Scholar
  • 10 Singh N, Puzhankara L, Kedlaya MN, Ramanarayanan V. Effectiveness of tea tree oil versus chlorhexidine in the treatment of periodontal diseases: a systematic review. Evid Based Dent 2022 ;10.1038/s41432-022-0259-6
    PubMedSearch in Google Scholar
  • 11 Casarin M, Pazinatto J, Santos RCV, Zanatta FB. Melaleuca alternifolia and its application against dental plaque and periodontal diseases: a systematic review. Phytother Res 2018; 32 (02) 230-242
    CrossrefPubMedSearch in Google Scholar
  • 12 Commar BC, Danelon M, Panitente PA. et al. Effect of glaze and chlorhexidine on physical and mechanical properties of bis-acryl resin: an in situ study. Polim Med 2022; 52 (02) 93-99
    CrossrefPubMedSearch in Google Scholar
  • 13 Rahman B, Alkawas S, Al Zubaidi EA, Adel OI, Hawas N. Comparative antiplaque and antigingivitis effectiveness of tea tree oil mouthwash and a cetylpyridinium chloride mouthwash: a randomized controlled crossover study. Contemp Clin Dent 2014; 5 (04) 466-470
    CrossrefPubMedSearch in Google Scholar
  • 14 World Medical Association. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA 2013; 310 (20) 2191-2194
    CrossrefPubMedSearch in Google Scholar
  • 15 Sankar A, Johnson SR, Beattie WS, Tait G, Wijeysundera DN. Reliability of the American Society of Anesthesiologists physical status scale in clinical practice. Br J Anaesth 2014; 113 (03) 424-432
    CrossrefPubMedSearch in Google Scholar
  • 16 Kulshrestha S, Khan S, Meena R, Singh BR, Khan AU. A graphene/zinc oxide nanocomposite film protects dental implant surfaces against cariogenic Streptococcus mutans. Biofouling 2014; 30 (10) 1281-1294
    CrossrefPubMedSearch in Google Scholar
  • 17 Manikandan S, Bhambal AM, Ratchambiga KS, Nithiela M, Swatheka JK, Sridarshini B. Comparative evaluation of the effect of 0.2% chlorhexidine, 2% LemonGrass Oil, and 2% tea tree oil mouth rinse on salivary pH: an in vivo study. J Pharm Bioallied Sci 2021; 13 (Suppl. 01) S757-S760
    CrossrefPubMedSearch in Google Scholar
  • 18 Dasanayake AP, Caufield PW, Cutter GR, Roseman JM, Köhler B. Differences in the detection and enumeration of mutans streptococci due to differences in methods. Arch Oral Biol 1995; 40 (04) 345-351
    CrossrefPubMedSearch in Google Scholar
  • 19 Lemos JA, Palmer SR, Zeng L. et al. The biology of Streptococcus mutans . Microbiol Spectr 2019; 7 (01) 10
    CrossrefPubMedSearch in Google Scholar