The Effect of Mixed Polymethylmethacrylate and Hydroxyapatite on Viability of Stem Cell from Human Exfoliated Deciduous Teeth and Osteoblast

Tania Saskianti; Shinta Purnamasari; Seno Pradopo; Alexander Patera Nugraha; Chiquita Prahasanti; Diah Savitri Ernawati; Masami Kanawa

doi:10.1055/s-0043-1768971

European Journal of Dentistry, Table of Contents

CC BY 4.0 · Eur J Dent 2024; 18(01): 314-320
DOI: 10.1055/s-0043-1768971

Original Article

The Effect of Mixed Polymethylmethacrylate and Hydroxyapatite on Viability of Stem Cell from Human Exfoliated Deciduous Teeth and Osteoblast

Authors

Tania Saskianti

¹Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Shinta Purnamasari

¹Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Seno Pradopo

¹Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Alexander Patera Nugraha

²Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Chiquita Prahasanti

³Department of Periodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Diah Savitri Ernawati

⁴Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
Masami Kanawa

⁵Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima, Japan

Abstract

Objectives Stem cell from human exfoliated deciduous teeth (SHED) has great potential for bone tissue engineering and cell therapy for regenerative medicine. It has been combined with biomaterials such as mixed of polymethylmethacrylate (PMMA) and hydroxyapatite (HA) as candidates for synthetic bone graft biomaterial. The aim of this study was to analyze the toxicity test of mixed PMMA-HA scaffold seeded with SHED and osteoblast in vitro.

Materials and Methods SHED was isolated from the pulp of noncarious deciduous teeth and osteoblast cells were cultured, and exposed to PMMA-HA scaffolds with three concentration groups: 20/80, 30/70, and 40/60 for 24 hours. Cytotoxicity test was performed by MTT assay to cell viability.

Statistical Analysis Data were analyzed using IBM SPSS Statistics 25, one-way analysis of variance followed by least significant difference test, considering the level of significance p-value less than 0.05

Results The percentage of SHED's viability was best in the PMMA-HA group with concentrations of 20/80, followed by 30/70, and 40/60 with 87.03, 75.33, and 65.79%, respectively. The percentage of osteoblast cell's viability was best in the PMMA-HA group with concentrations of 20/80, followed by 30/70, and 40/60 with 123.6, 108.36, and 93.48%, respectively.

Conclusions Mixed PMMA-HA was not toxic for the SHED and osteoblast. This characteristic is the initial requirement to be proposed as an alternative material for healing alveolar bone defects. In vivo animal research is mandatory to confirm the use of PMMA-HA on the alveolar defect model.

Keywords

viability - medicine - polymethylmethacrylate - hydroxyapatite - stem cell from human exfoliated deciduous teeth - osteoblast

Full Text

References

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