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CC BY 4.0 · Eur J Dent 2024; 18(01): 314-320
DOI: 10.1055/s-0043-1768971
Original Article

The Effect of Mixed Polymethylmethacrylate and Hydroxyapatite on Viability of Stem Cell from Human Exfoliated Deciduous Teeth and Osteoblast

Tania Saskianti
1   Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Shinta Purnamasari
1   Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Seno Pradopo
1   Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Alexander Patera Nugraha
2   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Chiquita Prahasanti 
3   Department of Periodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Diah Savitri Ernawati
4   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Masami Kanawa
5   Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima, Japan
› Author Affiliations Funding Research reported in this publication was supported by the Hibah Riset Mandat Kolaborasi Mitra Luar Negeri (KLN) Research Grant 2021 (No: 792/UN3.15/PT/2021) from Airlangga University Research and Community Service Institute.
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Abstract

Objectives Stem cell from human exfoliated deciduous teeth (SHED) has great potential for bone tissue engineering and cell therapy for regenerative medicine. It has been combined with biomaterials such as mixed of polymethylmethacrylate (PMMA) and hydroxyapatite (HA) as candidates for synthetic bone graft biomaterial. The aim of this study was to analyze the toxicity test of mixed PMMA-HA scaffold seeded with SHED and osteoblast in vitro.

Materials and Methods SHED was isolated from the pulp of noncarious deciduous teeth and osteoblast cells were cultured, and exposed to PMMA-HA scaffolds with three concentration groups: 20/80, 30/70, and 40/60 for 24 hours. Cytotoxicity test was performed by MTT assay to cell viability.

Statistical Analysis Data were analyzed using IBM SPSS Statistics 25, one-way analysis of variance followed by least significant difference test, considering the level of significance p-value less than 0.05

Results The percentage of SHED's viability was best in the PMMA-HA group with concentrations of 20/80, followed by 30/70, and 40/60 with 87.03, 75.33, and 65.79%, respectively. The percentage of osteoblast cell's viability was best in the PMMA-HA group with concentrations of 20/80, followed by 30/70, and 40/60 with 123.6, 108.36, and 93.48%, respectively.

Conclusions Mixed PMMA-HA was not toxic for the SHED and osteoblast. This characteristic is the initial requirement to be proposed as an alternative material for healing alveolar bone defects. In vivo animal research is mandatory to confirm the use of PMMA-HA on the alveolar defect model.

Keywords

viability - medicine - polymethylmethacrylate - hydroxyapatite - stem cell from human exfoliated deciduous teeth - osteoblast


Publication History

Article published online:
19 June 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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