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The Peroxidative Changes in Rat Brain Tissue Homogenates by Vitamin C and Deferoxamine against Vanadium-Stimulated Lipid PeroxidationFunding This study was supported by Department of Biochemistry, University of Benghazi, Libya.
Objectives Vanadium (V) metal induces lipid peroxidation (LPO) and this has been proposed as a cause for its neurotoxicity.
Aim This study aimed to evaluate the effects of vitamin C (VC) and deferoxamine (DF) against V-induced LPO in brain tissue homogenates in vitro.
Methods Male Sprague-Dawley rats were used. Brains were removed and dissected into hypothalamus, hippocampus, brain stem, and medulla pons. They were homogenized in150mM potassium chloride (KCl), and incubated for 1 hour with V, VC, and DF in a micromolar concentration of 20 and 100. Aliquots were used for the estimation of LPO in spectrophotometer. Data analysis were done by one-way analysis of variance.
Results V exposure (20 and 100μM) demonstrated statistically significant (p < 0.001) enhancement of LPO (average increase with 20μMV was by +105% and with 100μMV was by +130%), respectively, in brain tissue homogenates compared with water controls. Hypothalamus exhibited maximum enhancement (average increase with 20μMV was by +145% and with 100μMV was by +175%, respectively), in LPO than other regions. Coexposure of brain tissue homogenates to V + VC (20 and 100μM) further accelerated the LPO (+24% and +16%, respectively) compared with V alone. Brain stem exhibited highest increases (+54% with 20μMV and +21% with 100μMV; p < 0.001), respectively. V-induced oxidative consequences were remarkably inhibited (average -55%; p < 0.001) by DF + V (20μM + 100μM) exposure. Hypothalamus and medulla pons exhibited inhibition, by −66% and −60% (p < 0.001) respectively.
Conclusion V exposure in vitro resulted in oxidative damage with significant regional variations in brain tissue homogenates. VC is pro-oxidative in vitro reactions and DF chelates V-ion moiety.
The Limitation of This Study
The present investigation should have included measurement of antioxidant enzymes in brain tissues as a further extension of this study.
Article published online:
24 April 2023
© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
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