CC BY 4.0 · Chinese medicine and natural products 2023; 03(01): e41-e50
DOI: 10.1055/s-0043-1764132
Original Article

Wenyang-Yiqi Granule Suppresses Oxygen-Glucose Deprivation-Induced Cardiomyocyte Autophagy Through Mammalian Target of Rapamycin Activation in H9c2 Cells

Shuibo Gao
1   Laboratory of Cell Imaging, Second School of Clinical Medicine, Henan University of Chinese Medicine, Zhengzhou, Henan, China
,
Xiaofang Yu
2   Institute of Cardiovascular Disease, Henan University of Chinese Medicine, Zhengzhou, Henan, China
,
Lihua Han
2   Institute of Cardiovascular Disease, Henan University of Chinese Medicine, Zhengzhou, Henan, China
,
Hong Wu
1   Laboratory of Cell Imaging, Second School of Clinical Medicine, Henan University of Chinese Medicine, Zhengzhou, Henan, China
2   Institute of Cardiovascular Disease, Henan University of Chinese Medicine, Zhengzhou, Henan, China
› Author Affiliations
Funding This work was funded by the National Natural Science Foundation of China (81373610, 81673800).

Abstract

Background Wenyang-Yiqi Granule (WYYQ) is a four-component herbal formula, widely used to treat heart failure in China. It is known to regulate autophagy, but the mechanism(s) are unknown.

Methods H9c2 cells were treated with WYYQ for 24 hours prior to oxygen-glucose deprivation (OGD). Expressions of the autophagy markers Beclin-1 and light chain 3 (LC3) were evaluated via quantitative polymerase chain reaction analysis. Protein levels of Beclin-1, LC3, p62, and mammalian targets of rapamycin (mTOR) were determined by Western blot analysis. Transmission electron microscopy was used to explore the effects of WYYQ on autophagosome formation.

Results Treatment with WYYQ dramatically restrained OGD-induced autophagy, which was characterized by an inhibition of Beclin-1 and increased LC3 mRNA expression. In addition, WYYQ decreased the expression of Beclin-1 and the ratio of LC3-II/LC3-I; however, the abundance of p62 was enhanced at the protein level. Manipulation of the LC3-II/LC3-I ratio, p62 abundance, and autophagosome formation in response to WYYQ were associated with mTOR activity.

Conclusions These findings show that WYYQ plays a protective role during hypoxic-ischemic stress through the suppression of excessive autophagy, which may be partially explained by its effects on mTOR. These data provide novel insight into the cardioprotective effects of WYYQ during cardiomyocyte autophagy.

CRediT Authorship Contribution Statement

L.H. and H.W. were responsible for conceptualization, methodology, and formal analysis. S.G., X.Y., and H.W. were responsible for funding acquisition, writing original draft, and writing—review and editing.




Publication History

Received: 07 September 2022

Accepted: 20 October 2022

Article published online:
14 March 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 
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