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Drug Res (Stuttg) 2018; 68(06): 344-348
DOI: 10.1055/s-0043-123466
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Solvent Extraction and GC-MS Analysis of Sesame Seeds for Determination of Bioactive Antioxidant Fatty Acid/Fatty Oil Components

Abdul Qadir
1   Faculty of Pharmacy, Integral University, Lucknow, India
,
Athar Ali
2   Centre for Transgenic Plant Development, Department of Biotechnology, Hamdard University, New Delhi, India
,
Muhammad Arif
1   Faculty of Pharmacy, Integral University, Lucknow, India
,
Abdulmohsen H. Al-Rohaimi
3   College of Pharmacy, Al-Dawadmi Campus, Saqra University, Riyadh, KSA
,
Satya Prakash Singh
1   Faculty of Pharmacy, Integral University, Lucknow, India
,
Usama Ahmad
1   Faculty of Pharmacy, Integral University, Lucknow, India
,
Mohd Khalid
1   Faculty of Pharmacy, Integral University, Lucknow, India
,
Arun Kumar
1   Faculty of Pharmacy, Integral University, Lucknow, India
› Author Affiliations
Further Information

Publication History

received 01 August 2017

accepted 31 October 2017

Publication Date:
12 December 2017 (online)

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Abstract

The seed kernels of Sesamum indicum L. (family: Pedaliaceae) were extracted with ethanol and yield of components determined by Gas Chromatography/Mass Spectrometry (GC/MS). The free radical scavenging activities of ethanolic extract against1, 1-Diphenyl-2-picrylhydrazyl (DPPH) were determined by UV spectrophotometer at 517 nm. Phytochemical screening revealed the presence of numerous bioactive compounds including steroids, phenolic, terpenoids, fatty acids and different types of ester compounds. The ethanolic extract was purified and analyzed by GC MS.The prevailing compounds found in ethanolic extract were Carvacrol (0.04%),Sesamol (0.11%), 4-Allyl-2-methoxy-phenol(0.04%),Palmitic acid (1.08%), cis-9-Hexadecenal (85.40%), Lineoleoyl chloride (0.52%), Palmitic acid β-monoglyceride (0.40%), Dihydro-aplotaxene (0.61%), Oleoyl chloride (1.11%), (+)-Sesamin (4.73%), 1,3-Benzodioxole, 5-[4-(1,3-benzodioxol-5-yloxy)tetrahydro-1 H,3 H-furo [3,4-c]furan-1-yl], [1 S-(1,3,4,6α.), (2.01%)], 6-Nitrocholest-5-en-3-yl acetate (0.22%), Ergost-5-en-3β-ol (2.35%) and 24-Propylidenecholesterol (0.16%). The presence ofsaturated and unsaturated fatty acids in ethanolicextract justifies the use of this plant to treat many ailments in folk and traditional medicine. Ethanolic extract have shown significant antioxidant activity(IC50120.38±2.8 µg/ml). The presence of phenolic (Sesamol), lignin (Sesamin) compounds and unsaturated fatty acids are reported as possible contributor for antioxidantactivity of seed extract.

Key words

Sesamum indicum - Lignan - Sesamol - total antioxidant capacity - DPPH assay and GC/MS
 

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