Effect of Melatonin Supplementation on Antioxidant Status and DNA Damage in High Intensity Trained Athletes

Manuel Ortiz-Franco; Elena Planells; Bartholomé Quintero; Dario Acuña-Castroviejo; Iryna Rusanova; Germaine Escames; Jorge Molina-López

doi:10.1055/s-0043-119881

International Journal of Sports Medicine, Table of Contents

Int J Sports Med 2017; 38(14): 1117-1125
DOI: 10.1055/s-0043-119881

Nutrition

Effect of Melatonin Supplementation on Antioxidant Status and DNA Damage in High Intensity Trained Athletes

Manuel Ortiz-Franco

¹Department of Physiology, Faculty of Pharmacy, Campus de la Cartuja, University of Granada, Granada, Spain

²Institute of Nutrition and Food Technology, Biomedical Research Center, Health Campus, Avd. del Conocimiento, Granada, Spain

,

Elena Planells

¹Department of Physiology, Faculty of Pharmacy, Campus de la Cartuja, University of Granada, Granada, Spain

²Institute of Nutrition and Food Technology, Biomedical Research Center, Health Campus, Avd. del Conocimiento, Granada, Spain

,

Bartholomé Quintero

³Department of Physical Chemistry, Faculty of Pharmacy, Campus de la Cartuja, University of Granada. Granada, Spain

,

Dario Acuña-Castroviejo

⁴Department of Physiology. Faculty of Medicine, Campus de la Cartuja, University of Granada, Granada, Spain

⁵Institute of Biotechnology, Biomedical Research Center, Health Campus, Avd. del Conocimiento, Granada, Spain

,

Iryna Rusanova

⁴Department of Physiology. Faculty of Medicine, Campus de la Cartuja, University of Granada, Granada, Spain

⁵Institute of Biotechnology, Biomedical Research Center, Health Campus, Avd. del Conocimiento, Granada, Spain

,

Germaine Escames

⁴Department of Physiology. Faculty of Medicine, Campus de la Cartuja, University of Granada, Granada, Spain

⁵Institute of Biotechnology, Biomedical Research Center, Health Campus, Avd. del Conocimiento, Granada, Spain

,

Jorge Molina-López

¹Department of Physiology, Faculty of Pharmacy, Campus de la Cartuja, University of Granada, Granada, Spain

²Institute of Nutrition and Food Technology, Biomedical Research Center, Health Campus, Avd. del Conocimiento, Granada, Spain

› Author Affiliations

Abstract

The aim of the study was to evaluate the effect of melatonin supplementation on antioxidant capacity and DNA damage in high intensity interval training (HIIT) athletes. A 2-week randomised, double-blinded, placebo-controlled trial with two groups was conducted. Placebo (PG) and melatonin (MG) (20 mg/d) athletes were monitored over a two-week period of HIIT and strength training. The total antioxidant capacity (TAC) and the glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were analysed in blood samples. DNA damage was measured in isolated lymphocytes by comet assay prior to and immediately after exercise. The supplementation increased plasma melatonin levels in the melatonin-treated group (p<0.05) after two weeks of intervention. Analysis of antioxidant status indicated higher (p<0.05) TAC and GPx in MG than PG post-intervention. No differences were found in SOD enzyme activity. DNA damage was diminished in MG (p<0.05) compared to PG in post-training conditions. Antioxidant status was associated with DNA damage (r=-0.679; p=0.047) in the melatonin-treated athletes. The present study suggest that melatonin supplementation improves antioxidant status and may prove to have beneficial effects preventing DNA damage induced by high intensity training.

Key words

Melatonin - antioxidant status - DNA damage - comet assay - high-intensity interval training

Full Text

References

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