Exp Clin Endocrinol Diabetes 2018; 126(06): 371-378
DOI: 10.1055/s-0043-118667
© Georg Thieme Verlag KG Stuttgart · New York

Relationship between Oxidative Stress, Inflammation and Dyslipidemia with Fatty Liver Index in Patients with Type 2 Diabetes Mellitus

Aleksandra Klisic
1   Primary Health Care Center, Podgorica, Montenegro
Aleksandra Isakovic
2   Institute of Medical and Clinical Biochemistry, University of Belgrade – School of Medicine, Belgrade, Serbia
Gordana Kocic
3   Department of Medical Biochemistry, University of Nis – School of Medicine, Nis, Serbia
Nebojsa Kavaric
1   Primary Health Care Center, Podgorica, Montenegro
Milovan Jovanovic
1   Primary Health Care Center, Podgorica, Montenegro
Elvir Zvrko
4   Clinical Center of Montenegro, Podgorica, Montenegro
Verica Skerovic
4   Clinical Center of Montenegro, Podgorica, Montenegro
Ana Ninic
5   Department of Medical Biochemistry, University of Belgrade - Faculty of Pharmacy, Belgrade, Serbia
› Author Affiliations
Further Information

Publication History

received 02 June 2017
revised 18 July 2017

accepted 21 August 2017

Publication Date:
11 September 2017 (online)


Introduction/Aim Considering the high prevalence of non-alcoholic fatty liver disease (NAFLD) in individuals with type 2 diabetes mellitus (DM2), we aimed to investigate the potential benefit of determining markers of oxidative stress, inflammation and dyslipidemia for prediction of NAFLD, as estimated with fatty liver index (FLI) in individuals with DM2.

Methods A total of 139 individuals with DM2 (of them 49.9% females) were enrolled in cross-sectional study. Anthropometric and biochemical parameters, as well as blood pressure were obtained. A FLI was calculated.

Results Multivariate logistic regression analysis showed that high density lipoprotein cholesterol (HDL-c) and malondialdehyde (MDA) were independent predictors of higher FLI [Odds ratio (OR)=0.056, p=0.029; and OR=1.105, p=0.016, respectively]. In Receiver Operating Characteristic curve analysis, the addition of fatty liver risk factors (e. g., age, gender, body height, smoking status, diabetes duration and drugs metabolized in liver) to each analysed biochemical parameter [HDL-c, non-HDL-c, high sensitivity C-reactive protein (hsCRP), MDA and advanced oxidant protein products (AOPP)] in Model 1, increased the ability to discriminate patients with and without fatty liver [Area under the curve (AUC)=0.832, AUC=0.808, AUC=0.798, AUC=0.824 and AUC=0.743, respectively]. Model 2 (which included all five examined predictors, e. g., HDL-c, non-HDL-c, hsCRP, MDA, AOPP, and fatty liver risk factors) improved discriminative abilities for fatty liver status (AUC=0.909). Even more, Model 2 had the highest sensitivity and specificity (89.3% and 87.5%, respectively) together than each predictor in Model 1.

Conclusion Multimarker approach, including biomarkers of oxidative stress, dyslipidemia and inflammation, could be of benefit in identifying patients with diabetes being at high risk of fatty liver disease.