Drug Res (Stuttg)
DOI: 10.1055/s-0043-117612
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Lactobacilus Delbrueckii subsp. Bulgaricus Modulates the Secretion of Th1/Th2 and Treg Cell-Related Cytokines by PBMCs from Patients with Atopic Dermatitis

Abdolkarim Sheikhi1, 2, Hojjat Giti3, Mohammad Reza Heibor1, Abdollah Jafarzadeh4, Mansour Shakerian5, Narges Baharifar1, Fereidon Niruzad6, Ali Sadeghi Moghaddam7, Parviz Kokhaei8, Mohammad Baghaeifar5
  • 1Dpartment of Immunology, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran
  • 2Cellular and Molecular Immunology Research laboratory, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran
  • 3Tehran Chemie Pharmaceutical Company, Tehran, Iran
  • 4Department of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran
  • 5Pegah Khuzestan Pasturized Milk co., Dezful, Iran
  • 6Department of Pediatric Medicine, Ganjavian Hospital, Dezful University of Medical Sciences, Dezful, Iran
  • 7Faculty of Nursing and Midwifery, Dezful University of Medical Sciences, Dezful, Iran
  • 8Cancer Research Center, Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran
Further Information

Publication History

received 29 March 2017

accepted 24 July 2017

Publication Date:
12 September 2017 (eFirst)

Abstract

Background Atopic dermatitis (AD) is an inflammatory skin disease which may be due to the imbalance between Th1-, Th2 and Treg cell-related immune responses. Evidences suggest that appropriate stimulation with probiotics may correct the skewed immune response in children with AD. The aim was to determine the effects of the yogurt culture lactobacillus Bulgaricus on the secretion of Th1/Th2/Treg type cytokines by PBMCs from children with AD.

Methods L. Bulgaricus was cultivated on MRS broth. The PBMCs from 20 children with AD were separated by Ficoll-Hypaque centrifugation and co-cultured with different concentrations of UV killed bacteria in RPMI-1640 plus 10% FCS for 48/72 h. The levels of IL-10, IL-4, IL-12 and IFN-γ were measured in supernatant of PBMCs by ELISA.

Results L. Bulgaricus significantly up-regulated the secretion of IL-10, IL-12 and IFN-γ, whereas decreased the secretion of IL-4 by PBMCs at both incubation times 48 h/72 h and both bacteria:PBMCs ratios 100:1/50:1, compared to control (p<0.05). There were no significant differences between incubation times 48 h and 72 h regarding the secretion levels of IL-12, IFN-γ and IL-4. However, the secretion of IL-10 by L. Bulgaricus-stimulated PBMCs at incubation time 72 h and in the presence of bacteria:PBMCs ratio 100:1 was significantly higher than in incubation time 48 h and in the presence of bacteria:PBMCs ratio 50:1 (P<0.000 and P<0.00, respectively).

Conclusion These data show that L. Bulgaricus may modulate the secretion of Th1-, Th2-Treg-related cytokines in AD patients. Therefore, the possible potential therapeutic of L. Bulgaricus for treatment of AD should be consider in further investigation.