Effects of Cobalamin (Vitamin B12) on Gentamicin Induced Nephrotoxicity in Rat

Saeed Hajihashemi; Zeinab Hamidizad; Ali Rahbari; Fatemeh Ghanbari; Zohre Aghaee Motealeghi

doi:10.1055/s-0043-117418

Drug Research, Table of Contents

Drug Res (Stuttg) 2017; 67(12): 710-718
DOI: 10.1055/s-0043-117418

Original Article

Effects of Cobalamin (Vitamin B₁₂) on Gentamicin Induced Nephrotoxicity in Rat

Saeed Hajihashemi

¹Assistant professor of Physiology, Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran

,

Zeinab Hamidizad

²MSc in Physiology, Arak University of Medical Sciences, Arak, Iran

,

Ali Rahbari

³Assistant Professor of Pathology, Department of Pathology, Arak University of Medical Sciences, Arak, Iran

,

Fatemeh Ghanbari

⁴Assistant Professor of Pharmacology, Department of Pharmacology, Islamic Azad University of Arak, Arak, Iran

,

Zohre Aghaee Motealeghi

²MSc in Physiology, Arak University of Medical Sciences, Arak, Iran

› Author Affiliations

Abstract

Background The main side effect of gentamicin is nephrotoxicity. The effect of cobalamin (Cob) was investigated on gentamicin nephrotoxicity in rats.

Methods Renal injury induced by i.p. injection of gentamicin (100 mg/kg) for 8 consecutive days. Cobalamin (6 mg/kg/day, i.p) treatment was done for 8 consecutive days as co-treatment and post-treatment protocol.

Results Cobalamin significantly increased creatinine clearance levels and renal blood flow which were reduced by gentamicin. Also, cobalamin significantly improved serum electrolytes (sodium and potassium) levels which were disturbed by gentamicin. Cobalamin significantly compensated deficits in the antioxidant defense mechanisms, suppressed lipid per oxidation and ameliorated renal tissue damage mediated by gentamicin.

Conclusion The results of the current study indicated that cobalamin effectively protected the kidney tissue against gentamicin induced acute nephrotoxicity in rats. The antioxidant and anti-inflammatory activities can be supposed the main factors responsible for the nephroprotective effect of cobalamin.

Key words

cobalamin (vitamin B12) - gentamicin induced nephrotoxicity - rat

Full Text

References

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