Drug Res (Stuttg) 2017; 67(12): 710-718
DOI: 10.1055/s-0043-117418
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Effects of Cobalamin (Vitamin B12) on Gentamicin Induced Nephrotoxicity in Rat

Saeed Hajihashemi
Assistant professor of Physiology, Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
Zeinab Hamidizad
MSc in Physiology, Arak University of Medical Sciences, Arak, Iran
Ali Rahbari
Assistant Professor of Pathology, Department of Pathology, Arak University of Medical Sciences, Arak, Iran
Fatemeh Ghanbari
Assistant Professor of Pharmacology, Department of Pharmacology, Islamic Azad University of Arak, Arak, Iran
Zohre Aghaee Motealeghi
MSc in Physiology, Arak University of Medical Sciences, Arak, Iran
› Author Affiliations
Further Information

Publication History

received 03 July 2017

accepted 20 July 2017

Publication Date:
12 September 2017 (eFirst)


Background The main side effect of gentamicin is nephrotoxicity. The effect of cobalamin (Cob) was investigated on gentamicin nephrotoxicity in rats.

Methods Renal injury induced by i.p. injection of gentamicin (100 mg/kg) for 8 consecutive days. Cobalamin (6 mg/kg/day, i.p) treatment was done for 8 consecutive days as co-treatment and post-treatment protocol.

Results Cobalamin significantly increased creatinine clearance levels and renal blood flow which were reduced by gentamicin. Also, cobalamin significantly improved serum electrolytes (sodium and potassium) levels which were disturbed by gentamicin. Cobalamin significantly compensated deficits in the antioxidant defense mechanisms, suppressed lipid per oxidation and ameliorated renal tissue damage mediated by gentamicin.

Conclusion The results of the current study indicated that cobalamin effectively protected the kidney tissue against gentamicin induced acute nephrotoxicity in rats. The antioxidant and anti-inflammatory activities can be supposed the main factors responsible for the nephroprotective effect of cobalamin.