Drug Res (Stuttg)
DOI: 10.1055/s-0043-116947
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Multicomponent Synthesis of Some Molecular Hybrid Containing Thiazole Pyrazole as Apoptosis Inducer

Parvin Kumar1, Meenakshi Duhan1, Kulbir Kadyan1, Jitender Kumar Bhardwaj2, Priyanka Saraf2, Meenu Mittal2
  • 1Department of Chemistry, Kurukshetra University Kurukshetra-136119
  • 2Reproductive Physiology Laboratory, Department of Zoology,Kurukshetra University Kurukshetra-136119
Further Information

Publication History

received 07 June 2017

accepted 14 July 2017

Publication Date:
14 September 2017 (eFirst)

Abstract

The present study describes a multicomponent synthesis of molecular hybrid containing pyrazole, thiazole moiety using hydrazone as a linker, which have been synthesized by condensation of 1-phenyl-3-(aryl)-1H-pyrazole-4-carbaldehydes 1a-b, thiosemicarbazide and α-bromoketones 2a-c.The target hybrid compounds, 1-((1-phenyl-3-aryl-1H-pyrazole-4-yl)methylene)-2-(4-arylthiazole-2-yl)hydrazine 3a-f are characterized by 1H-NMR, 13C NMR, FT-IR and mass. Apoptosis inducing ability and cytotoxic nature of all the hybrid compounds having thiazole, pyrazole and hydrazone were assessed by using biological assays viz morphological, fluorescence and tunel assays on granulosa cells of ovarian antral follicles of goat (Capra hircus) in vitro. Apoptosis was recognized and quantified using differential staining of ethidium bromide and acridine orange where apoptotic cells exhibited red fluorescence and live normal cells with intact cell membrane and normal nucleus displayed bright green fluorescence. Among the tested compounds, compound 3e and 3b showed the maximum potency to induce apoptosis with percentage of apoptosis 25.61±2.95and 23.45±1.46 respectively followed by 3f (20.95±0.40) and 3d (20.44±1.60) in comparison with control (5.14±0.44).

Supplementary Material