Ginger Extract Modulates the Expression of Chemokines CCL20 and CCL22 and Their Receptors (CCR6 and CCR4) in the Central Nervous System of Mice with Experimental Autoimmune Encephalomyelitis

Abdollah Jafarzadeh; Zahra Arabi; Rayhaneh Ahangar-Parvin; Marziyeh Mohammadi-Kordkhayli; Maryam Nemati

doi:10.1055/s-0043-113455

Drug Research, Inhaltsverzeichnis

Drug Res (Stuttg) 2017; 67(11): 632-639
DOI: 10.1055/s-0043-113455

Original Article

Ginger Extract Modulates the Expression of Chemokines CCL20 and CCL22 and Their Receptors (CCR6 and CCR4) in the Central Nervous System of Mice with Experimental Autoimmune Encephalomyelitis

Abdollah Jafarzadeh

¹Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

²Department of Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

³Department of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran

,

Zahra Arabi

²Department of Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

,

Rayhaneh Ahangar-Parvin

²Department of Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

,

Marziyeh Mohammadi-Kordkhayli

⁴Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

,

Maryam Nemati

³Department of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran

⁵Department of Laboratory Sciences, Para-Medicine School, Kerman University of Medical Sciences, Kerman, Iran

› Institutsangaben

Abstract

Background Chemokines facilitate the leukocytes infiltration into the central nervous system (CNS) which is an essential step in the pathogenesis of multiple sclerosis. Ginger has also a broad anti-inflammatory properties. The aim was to evaluate the effects of ginger extract on the expression of CCL20 and CCL22 and their receptors (CCR6 and CCR4, respectively) in experimental autoimmune encephalomyelitis (EAE).

Material and Methods Female C57BL/6 mice used for EAE induction by immunization with myelin oligodendroglial glycoprotein. Then, the EAE mice were treated with PBS or ginger extract, from day +3 to +30. At day 31, mice were scarified and the expression of CCL20 and CCL22 and their receptors in the spinal cord measured using real time-PCR.

Results The expression of CCL20, CCL22 and CCR4 in the spinal cord of PBS-administrated EAE mice was significantly higher than healthy group (P<0.04, P<0.05 and P<0.02, respectively). In 200- and 300 mg/kg ginger extract-treated EAE mice, the expression of CCL20, CCL22 and CCR4 were significantly reduced as compared with PBS-administrated EAE group (P<0.04, P<0.01 and P<0.002 for 200 mg/kg ginger extract and P<0.01, P<0.005 and P<0.004 for 300 mg/kg ginger extract, respectively). The CCR6 expression in EAE mice treated with 200- or 300 mg/kg ginger extracts was lower than PBS-administrated EAE mice (P<0.01 and P=0.07, respectively).

Conclusion Treatment of EAE mice with ginger extract down-regulate the expression of CCL20 and CCL22 and their receptors in EAE mice. The possible therapeutic potential of ginger for treatment of MS can be considered in future investigations.

Key words

experimental autoimmune encephalomyelitis - ginger extract - CCL20 - CCL22 - CCR4 - CCR6

Volltext

Referenzen

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