Exp Clin Endocrinol Diabetes 2017; 125(10): 684-689
DOI: 10.1055/s-0043-110054
© Georg Thieme Verlag KG Stuttgart · New York

Nitric Oxide Plasma Level as a Barometer of Endothelial Dysfunction in Factory Workers

Seiko Miyata1, Akiko Noda2, Yuki Hara3, Jun Ueyama3, Kiyoyuki Kitaichi4, Takaaki Kondo3, Yasuo Koike2
  • 1Department of Sleep Medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • 2Department of Biomedical Sciences, Chubu University Graduate School of Life and Health Sciences, Kasugai, Japan
  • 3Department of Pathophysiological Laboratory Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan
  • 4Laboratory of Pharmaceutics, Department of Medical Pharmaceutics, Gifu Pharmaceutical University, Gifu, Japan
  • 5Department of Clinical Engineering, Chubu University College of Life and Health Sciences, Kasugai, Japan
Further Information

Publication History

received 26 November 2016
revised 26 April 2017

accepted 28 April 2017

Publication Date:
27 July 2017 (eFirst)


Objective Nitric oxide (NO) plays a key role in the regulation of vascular tone and is known as one of the key markers of endothelial dysfunction. We investigated the relationship between NO and risk factors of lifestyle-related disease in factory workers.

Methods Our study included 877 factory workers presenting hypertension, dyslipidemia and type 2 diabetes. Oxidated forms of NO, NO2-/NO3- (NOx) plasma concentrations were measured using a colorimetric method.

Results NOx plasma levels in patients with lifestyle-related disease were significantly lower than those in the controls. The brachial-ankle pulse wave velocity (baPWV) measured in those patients was significantly greater than that of the controls. Multiple regression analysis revealed that LDL cholesterol was an independent risk factor for reducing NOx plasma concentrations. Interestingly, individuals with low NOx plasma concentrations were more likely to present type 2 diabetes compared to those with the highest plasma levels of NOx (odds ratio [OR] [95% confidence interval; CI]=3.65 [1.61–8.28], P=0.002, 2.67 [1.15–6.20], P=0.022, and 3.27 [1.43–7.48], P=0.005). Subjects with the lowest levels of plasma NOx were more likely to present dyslipidemia (OR [95% CI]=1.69 [1.13–2.53], P=0.01).

Conclusion Endothelial function evaluated with plasma NOx may be indicative of lifestyle-related diseases independently from the vascular function assessed using baPWV.