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Planta Med 2017; 83(14/15): 1149-1158
DOI: 10.1055/s-0043-109098
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Metabolite Profile and Antiproliferative Effects in HaCaT Cells of a Salix reticulata Extract[*]

Elisabetta Corradi
1   Division of Pharmaceutical Biology, University of Basel, Switzerland
,
Nadine Schmidt
2   Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, Switzerland
,
Nathalie Räber
1   Division of Pharmaceutical Biology, University of Basel, Switzerland
,
Maria De Mieri
1   Division of Pharmaceutical Biology, University of Basel, Switzerland
,
Matthias Hamburger
1   Division of Pharmaceutical Biology, University of Basel, Switzerland
,
Veronika Butterweck
2   Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, Switzerland
,
Olivier Potterat
1   Division of Pharmaceutical Biology, University of Basel, Switzerland
› Author Affiliations
Further Information

Publication History

received 31 January 2017
revised 03 April 2017

accepted 05 April 2017

Publication Date:
27 April 2017 (online)

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Abstract

Phenolic constituents of Salix reticulata (Salicaceae) and antiproliferative activity of an extract and individual compounds were investigated in immortalized human non-tumorigenic keratinocytes (HaCaT). A MeOH extract from aerial parts afforded several flavonoids, including luteolin and apigenin glycosides (2–5 and 9) and catechin (1), two procyanidin fractions, and the phenolic glucosides picein (6), triandrin (7), and salicortin (8). In an adenosine triphosphate assay, the MeOH extract reduced cell viability by approximately 60 % at a concentration of 100 µg/mL. Cell proliferation was assessed with a BrdU incorporation ELISA assay. The extract inhibited proliferation of HaCaT cells in a concentration-dependent manner, with approximately 50 % inhibition at 100 µg/mL. In time-lapse assays, the extract showed distinct inhibitory effects on cell migration at concentrations of 12.5, 25, and 50 µg/mL. The activity of selected constituents was also determined. Luteolin-7-O-β-glucuronide (3) significantly inhibited cell proliferation at concentrations of 10 and 50 µM. In contrast, luteolin-7-O-β-glucopyranoside (2) and a procyanidin fraction (P1) had only weak effects, while picein (6) and salicortin (8) did not affect cell proliferation. Luteolin-7-O-β-glucuronide (10 µM) and, to a lesser extent, the procyanidin fraction (10 µg/mL) also inhibited cell migration.

Key words

Salix reticulata - Salicaceae - flavone glycosides - procyanidins - phenolic glucosides - luteolin-7-O-β-glucuronide - antiproliferative activity - cell migration

* Dedicated to Professor Dr. Max Wichtl in recognition of his outstanding contribution to pharmacognosy research.


Supporting Information

    NMR data of luteolin-7-O-β-glucuronopyranoside (3) and luteolin-7-O-(6″-O-malonyl)-β-glucopyranoside (4), 1H NMR spectrum of 3, quasimolecular ions observed in HPLC-ESIMS analysis, and calibration curves of luteolin-7-O-β-glucopyranoside (2) and picein (6) are available as Supporting Information.

  • Supporting Information
 

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