Planta Med 2017; 83(10): 846-854
DOI: 10.1055/s-0043-104391
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Chamomile Flower, Myrrh, and Coffee Charcoal, Components of a Traditional Herbal Medicinal Product, Diminish Proinflammatory Activation in Human Macrophages

Cica Vissiennon
1  University of Leipzig, Medical Faculty, Institute of Medical Physics and Biophysics, Leipzig
3  REPHA GmbH Biologische Arzneimittel, Langenhagen
,
Dima Hammoud
1  University of Leipzig, Medical Faculty, Institute of Medical Physics and Biophysics, Leipzig
2  Institut Régional du Génie Industriel et Biotechnologiques et sciences appliquées (IRGIB-Africa), Cotonou, République du Bénin
,
Steffen Rodewald
4  University of Leipzig, Institute of Pharmacy, Leipzig
,
Karin Fester
4  University of Leipzig, Institute of Pharmacy, Leipzig
,
Karl-Heinz Goos
3  REPHA GmbH Biologische Arzneimittel, Langenhagen
,
Karen Nieber
4  University of Leipzig, Institute of Pharmacy, Leipzig
,
Jürgen Arnhold
1  University of Leipzig, Medical Faculty, Institute of Medical Physics and Biophysics, Leipzig
› Author Affiliations
Further Information

Publication History

received 24 August 2016
revised 31 January 2017

accepted 10 February 2017

Publication Date:
06 March 2017 (online)

Abstract

A traditional herbal medicinal product, containing myrrh, chamomile flower, and coffee charcoal, has been used in Germany for the relief of gastrointestinal complaints for decades. Clinical studies suggest its use in the maintenance therapy of inflammatory bowel disease. However, the pharmacological mechanisms underlying the clinical effects are not yet fully understood.

The present study aims to elucidate immunopharmacological activities of myrrh, chamomile flower, and coffee charcoal by studying the influence of each plant extract on gene expression and protein release of activated human macrophages.

The plant extracts effect on gene and protein expression of activated human monocyte-derived macrophages was investigated by microarray gene expression analysis and assessment of the release of pro- and anti-inflammatory mediators (TNFα, chemokine CXCL13, and interleukin-10) using an ELISA test system.

The extracts of myrrh, chamomile flower, and coffee charcoal influenced gene expression of activated human macrophages within the cytokine/chemokine signaling pathway. Particularly, chemokine gene expression was suppressed. Subsequently, the production of CXCL13 and, to a minor extent, cytokine TNFα was inhibited by all herbal extracts. Chamomile flower and coffee charcoal extracts enhanced interleukin-10 release from activated macrophages. The observed effects on protein release were comparable to the effect of budesonide, which decreased TNFα and CXCL13 and enhanced interleukin-10 release.

The components of the herbal medicinal product influence the activity of activated human macrophages on both gene and protein level. The induced alterations within chemokine/cytokine signaling could contribute to a positive effect on the immunological homeostasis, which is disturbed in patients with chronic intestinal inflammation.

Supporting Information

HPLC chromatograms and LC-MS data as well as hierarchical figures of gene ontological enrichment analysis and tables with expression values of differentially expressed genes are available as Supporting Information.