Bromelain Loading and Release from a Hydrogel Formulated Using Alginate and Arabic Gum

Janaína Artem Ataide; Letícia Caramori Cefali; Marcia de Araujo Rebelo; Lívia Genovez Spir; Elias Basile Tambourgi; Angela Faustino Jozala; Marco Vinícius Chaud; Edgar Silveira; Xiaochen Gu; Priscila Gava Mazzola

doi:10.1055/s-0043-102948

Planta Medica, Inhaltsverzeichnis

Planta Med 2017; 83(10): 870-876
DOI: 10.1055/s-0043-102948

Formulation and Delivery Systems of Natural Products

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Bromelain Loading and Release from a Hydrogel Formulated Using Alginate and Arabic Gum

Authors

Janaína Artem Ataide

¹Postgraduate Program in Medical Sciences, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil
Letícia Caramori Cefali

²Postgraduate Program in Biosciences and Technology of Bioactive Products, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil
Marcia de Araujo Rebelo

³Laboratory of Biomaterials & Nanotechnology, Universidade de Sorocaba (UNISO), Sorocaba, Brazil
Lívia Genovez Spir

⁴Faculty of Pharmaceutical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil
Elias Basile Tambourgi

⁵Chemical Engineering Systems Department, School of Chemical Engineering, State University of Campinas (UNICAMP), Campinas, Brazil
Angela Faustino Jozala

⁶Department of Technological and Environmental Processes, Universidade de Sorocaba (UNISO), Sorocaba, Brazil
Marco Vinícius Chaud

³Laboratory of Biomaterials & Nanotechnology, Universidade de Sorocaba (UNISO), Sorocaba, Brazil
Edgar Silveira

¹Postgraduate Program in Medical Sciences, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil

⁷Genetics and Biochemistry Institute, Uberlandia Federal University (UFU), Uberlandia, Brazil
Xiaochen Gu

⁸College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada
Priscila Gava Mazzola

⁴Faculty of Pharmaceutical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil

Abstract

An ideal wound dressing ensures a moist environment around the wound area and absorbs exudates from the wound surface. Topical application of bromelain to incised wounds has been shown to reprogram the wound microenvironment to promote effective tissue repair. Combining the characteristics of hydrogels and bromelain is therefore of great interest. Herein, we describe the development of a hydrogel, formulated using alginate and Arabic gum, for bromelain loading and release. The hydrogel formulation was evaluated using response surface methodology, considering the pH value and the concentration of alginate and Arabic gum. Bromelain loading and release were evaluated based on passive diffusion. Differential scanning calorimetry and Fourier transform infrared spectroscopy were performed to confirm bromelain immobilization in the hydrogel. The final hydrogel formulation had a swelling ratio of 227 % and incorporated 19 % of bromelain from a bromelain solution. Bromelain immobilization in the hydrogel was the result of hydrogen bond formation and was optimal at 4 °C after 4 h of contact. This evidence suggests that bromelain entrapment into a hydrogel is a promising strategy for the development of wound dressings that support the debridement of burns and wounds.

Key words

bromelain - hydrogel - alginate - Arabic gum - formulation development - Ananas comosus L. - Bromeliaceae

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Referenzen

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