Planta Med 2022; 88(15): 1497
DOI: 10.1055/s-0042-1759149
Poster Session I

Viscum album hydrogel: physico-chemical evaluation and antiproliferative assay

C Holandino
1   Universidade Federal Do Rio De Janeiro, Rio de Janeiro, Brazil
M Souza Rocha
1   Universidade Federal Do Rio De Janeiro, Rio de Janeiro, Brazil
J V da Costa Batista
2   Society for Cancer Research, Arlesheim, Switzerland
V Emerich Bucco de Campos
3   Univesidade Estadual do Rio de Janeiro, Rio de Janeiro, Brazil
A Passos Oliveira
1   Universidade Federal Do Rio De Janeiro, Rio de Janeiro, Brazil
S Baumgartner
2   Society for Cancer Research, Arlesheim, Switzerland
› Author Affiliations

Viscum album L. is a plant species, which clinical importance has been registered either as monotherapy or adjunct therapy for patients with cancer [1], [2]. Itʼs well known the use of different extracts in the V. album preparations that influence the wide diversity of metabolites detected in these pharmaceutical preparations [3], [4], [5]. The objective of this study was to evaluate the physico-chemical and antitumoral in vitro effect of a hydrogel containing ethanolic dry extract of V. album (host tree Abies alba). For this, dynamic light scattering (DLS), transmission and scanning electron microscopy (TEM, SEM), and Ultraviolet Spectrometry (UV) were applied. The antiproliferative and cellular features were evaluated by MTT and Giemsa stain, respectively, using non-tumor (L929) and tumor (SCC-25) cells. DLS showed particles with an average size of 256 ± 7 nm (0 day) and 258 ± 1 nm, after 30 days storage. The TEM and SEM analyses revealed well-dispersed spherical droplets, with regular borders and nanometer sizes (300 – 500 nm). Flavonoids content in equivalents of rutin was 13.22 ± 0.02 mg/g. MTT showed IC50 of 333.40 µg/mL and 1433 µg/mL for SCC-25 and L929, respectively, with 4.3 of selectivity index for the tumor in relation to the non-tumor cell. Giemsa stain showed intensive damage in tumoral cells, such as: swelling and lysis, intensive vacuolization, chromatin condensation and fragmentation. In conclusion, the Viscum album hydrogel spontaneously formed stable nanoparticles and the formulation has a promising antitumoral activity highlighted by the tumor selective index and cellular damage.

Publication History

Article published online:
12 December 2022

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