Planta Med 2022; 88(15): 1477
DOI: 10.1055/s-0042-1759105
Poster Session I

Comparison of antioxidant activity of Trifolium pratense L. extracts prepared using β- or γ-cyclodextrin-assisted extractions

JA Kazlauskaite∗
1   Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, Kaunas, Lithuania
2   Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Kaunas, Lithuania
,
J Bernatoniene
1   Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, Kaunas, Lithuania
2   Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Kaunas, Lithuania
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Red clover is valued for its positive influence of isoflavones on health and its potential use in preventing and treating chronic diseases [1], [2]. The right excipients, such as β- or γ cyclodextrin, can increase valuable phenolic compounds in extraction media thus obtaining antioxidant-rich extracts, which can be used in the pharmaceutical industry.

This study aimed to investigate and compare the total phenolic content and antioxidant activity of red clover aerial parts ethanolic extracts prepared using traditional and cyclodextrins-assisted extraction methods.

All the samples were prepared using three different methods: reflux (1); reflux combined with ultrasound processing for 10 min (2); reflux combined with ultrasound processing for 30 min (3). Compared with samples prepared using traditional methods without cyclodextrins (control samples C1-3), β-cyclodextrin (B1-3) increased total phenolic content by 11.77% and γ-cyclodextrin (G1-3) by 28.66% on average. The highest antioxidant activity (ABTS and DPPH) was detected in the samples G1: 433.122 ± 2.61 µg TE/g (ABTS) and G2: 12.55 ± 0.59 µg TE/g (DPPH). Using the post-column ABTS method, the highest effect was found in sample G1: 30.16 ± 1.21 mg TE/g. However, the highest reducing power activity was determined in the B3 sample prepared using β-cyclodextrin (191.92 ± 0.73 mg FE(II)/g).

Both cyclodextrin-assisted extraction methods are useful tools for obtaining antioxidant-rich extracts for the production of nutraceuticals. Nevertheless, γ-cyclodextrin-assisted method showed greater results compared to β-cyclodextrin.



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Artikel online veröffentlicht:
12. Dezember 2022

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