Antioxidant activity and GC-MS analysis of the components of aqueous leaf extract of Spathodea campanulata

The antioxidant capacity helps reduce the pro-oxidative state inflicted by reactive oxygen species (ROS) produced either from natural cell metabolisms or from external sources and increased in different chronic and degenerative diseases. Antioxidant compounds have received attention in recent years, particularly from medicinal plants. Several studies reported the bioactive compounds present in plants called phytochemicals produced in the secondary plant metabolism. Aim: To evaluate the antioxidant capacity and evaluate the main components in the aqueous extract. Material and Methods. Aqueous extract of leaves of Spathodea campanulata (AQESCL). The antioxidant activity was evaluated using the 1-1-dyphenyl-2-picrylhydrazyl (DPPH) method (Bonet and Brand-Williams, 1995). To identify the components present a gas chromatography-mass spectrometry on Agilent 7000C Triple Quad w/7890B GC/MS/MS system with an Agilent J&W GC Column was performed. Results. The AQELSC presented an EC₅₀ of 70.86 ± 9.67 g/Kg DPPH, with a Specific activity of 7.84 X 10 – 3 representing high antiradical efficiency. GC-MS analysis revealed the presence of 36 constituents. The major components present in the aqueous extracts were: 9-(3,3-Dimethyloxiran-2-yl)-2,7-dimethylnona-2,6-dien-1-ol: 14.97%, Cyclopentaneacetaldehyde, 2-formyl-3-methyl-α-methylene-: 11.13%, 2,2,6-Trimethyl-1-(3-methylbuta-1,3-dienyl)-7-oxabicyclo[4.1.0]heptan-3-ol, 14.19%, 1,5-Hexadien-3-ol, 3-methyl-6-(methylthio)-1-(2,6,6-trimethyl-1-cyclohexen-1-yl)-, (E,E)-: 6.77, and 6-Hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one 6.59, giving 53.95% of the components found. They have been related to different functions, including reducing oxidative stress. Conclusion. The AQESCL present high antioxidant activity due to the secondary metabolites present.

Acknowledgements

CIC-UMSNH-2022 for the partial support.

Keywords: Antioxidant activity, aqueous extract, GC-MS Analysis, Spathodea campanulata.

 

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Artikel online veröffentlicht:
12. Dezember 2022