Planta Med 2022; 88(15): 1462
DOI: 10.1055/s-0042-1759068
Poster Session I

Sustainable exploitation of Castanea sativa L. pruning by-products as a source of bioactive compounds with anti-inflammatory activity in intestinal cells

C Pozzoli∗
University of Milan – Department of Pharmacological and Molecular Sciences, via Balzaretti 9, 20133 Milan, Italy
,
E Sangiovanni
University of Milan – Department of Pharmacological and Molecular Sciences, via Balzaretti 9, 20133 Milan, Italy
,
M Fumagalli
University of Milan – Department of Pharmacological and Molecular Sciences, via Balzaretti 9, 20133 Milan, Italy
,
S Piazza
University of Milan – Department of Pharmacological and Molecular Sciences, via Balzaretti 9, 20133 Milan, Italy
,
N Maranta
University of Milan – Department of Pharmacological and Molecular Sciences, via Balzaretti 9, 20133 Milan, Italy
,
M Angarano
University of Milan – Department of Pharmacological and Molecular Sciences, via Balzaretti 9, 20133 Milan, Italy
,
A Magnavacca
University of Milan – Department of Pharmacological and Molecular Sciences, via Balzaretti 9, 20133 Milan, Italy
,
G Martinelli
University of Milan – Department of Pharmacological and Molecular Sciences, via Balzaretti 9, 20133 Milan, Italy
,
M DellʼAgli
University of Milan – Department of Pharmacological and Molecular Sciences, via Balzaretti 9, 20133 Milan, Italy
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Agronomic practices such as pruning of a chestnut tree (Castanea sativa Mill., [C. sativa]) produce a large amount of several by-products, such as wood and buds. These wastes are an interesting source of bioactive molecules, mainly phenolics compounds, mostly phenolic acids and ellagitannins.

Chestnut pericarp and episperm have been recently identified as a rich source of proanthocyanidins and showed a significant anti-inflammatory activity in gastric epithelial cells [1]. This research aimed therefore to investigate the potential anti-inflammatory and antioxidant activities of chestnut by-products in intestinal cells. Individual ellagitannins and total phenolic compounds were analyzed as well. Hydroalcoholic (H2O: EtOH 50 : 50) and aqueous extracts of C. sativa woods and buds were prepared. To study the anti-inflammatory effect, the extracts were assayed in an in vitro model of undifferentiated human intestinal Caco-2 cells (colonocytes) stimulated with IL-1β-IFNγ. Antioxidant activity of the extracts was evaluated through DPPH and ORAC assays.

Hydroalcoholic extracts of C. sativa woods and buds inhibited the NF-kB driven transcription and the release of CXCL-10 in a concentration-dependent manner, both induced by IL-1β-IFNγ. The aqueous and hydroalcoholic extracts exploited antioxidant activity (30 – 50 mmol Trolox eq/g) and contained bioactive molecules such as phenolic compounds (290 – 430 mg gallic acid equivalent/g) and ellagitannins.

C. sativa pruning wastes are highly rich in bioactive compounds and performed both antioxidant and anti-inflammatory activities in intestinal cells. This preliminary work contributes to the valorization of chestnut by-products and their possible recycle.

The authors declare no conflict of interest.



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Artikel online veröffentlicht:
12. Dezember 2022

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  • Reference

  • 1 Sangiovanni E, Piazza S, Vrhovsek U. et al. A bio-guided approach for the development of a chestnut-based proanthocyanidin-enriched nutraceutical with potential anti-gastritis properties. Pharmacological Research 2018; 134: 145-155
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