Planta Med 2022; 88(15): 1454
DOI: 10.1055/s-0042-1759046
Poster Session I

Human plasma metabolic changes induced by plant protein-enriched biscuits consumption via LC-triple-TOF employing DIA and LC-orbitrap using DDA

V Papaioannou∗
1   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimioupoli, Zografou, 15771, Athens, Greece
,
A Yanni
2   Laboratory of Chemistry-Biochemistry-Physical Chemistry of Foods, Department of Nutrition and Dietetics, Athens, Greece
,
V Karathanos
2   Laboratory of Chemistry-Biochemistry-Physical Chemistry of Foods, Department of Nutrition and Dietetics, Athens, Greece
,
M Halabalaki
1   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimioupoli, Zografou, 15771, Athens, Greece
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Protein-enriched foods have been shown to exert appetite-regulating properties¹. In this study, a wheat biscuit enriched with legume proteins was examined regarding its effects on weight loss and the metabolic profile of obese individuals. A 3-months, two-arm parallel dietary intervention of 80 overweight volunteers was conducted, in which half of the volunteers consumed daily a protein biscuit enriched, while the rest consumed a conventional wheat biscuit. Both groups were subjected to a weight loss diet.

The interventionʼs plasma samples were analyzed for 44 AA, incorporating a LC-triple-TOF system and the aTRAQ Kit. Moreover, the samples were subjected to metabolomic analysis with two LC-MS platforms, LC-triple-TOF employing SWATH acquisition mode (Data Independent Acquisition, DIA) and LC-Orbitrap using Data Depended Acquisition (DDA) mode, in order to compare the effectiveness of the instrumentations. In parallel, an investigation of the variations of the plasma metabolites before and after the intervention was carried out.

According to the study results, it was observed that the trial group presented a more considerable reduction in body mass index compared to the control group. Furthermore, changes were observed in the metabolic profile of the Individuals. For instance, obesity biomarkersʼ plasma concentrations, like aromatic AA, were reduced after dieting and protein supplementation.

Overall, functional foods supplemented with protein improve the metabolic responses related to appetite regulation. In contrast, both analytical platforms are proving to be powerful tools for metabolic profiling on complex samples and human intervention studies.

There is no conflict of interest. Funding by OREKTOSPRION. (T1EDK-03747)



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Artikel online veröffentlicht:
12. Dezember 2022

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