Planta Med 2022; 88(15): 1450
DOI: 10.1055/s-0042-1759037
Poster Session I

The metabolic profile of Anchusa officinalis L. differs according to its associated arbuscular mycorrhizal fungi

E Tsiokanos∗
1   Department of Pharmacy, Division of Pharmacognosy and Natural Product Chemistry, National and Kapodistrian University of Athens, Athens, Greece
,
A Cartabia
2   Applied Microbiology, Mycology, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Greece
,
N Tsafantakis
1   Department of Pharmacy, Division of Pharmacognosy and Natural Product Chemistry, National and Kapodistrian University of Athens, Athens, Greece
,
I Lalaymia
2   Applied Microbiology, Mycology, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Greece
,
A Termentzi
3   Laboratory of Toxicological Control of Pesticides, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, Athens, Greece
,
S Declerck
2   Applied Microbiology, Mycology, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Greece
,
N Fokialakis
1   Department of Pharmacy, Division of Pharmacognosy and Natural Product Chemistry, National and Kapodistrian University of Athens, Athens, Greece
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A recent study demonstrated the impact of the arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis MUCL41 833 on the metabolome of Anchusa officinalis [1]. However, it is not known whether AMFs belonging to the same genus impact the primary (PM) and secondary (SM) metabolites of A. officinalis in a similar way. Therefore, we tested the hypothesis that different strains of AMF affect the plant metabolome differently. Four AMF species, belonging to the genus Rhizophagus (R. irregularis MUCL41 833, R. intraradices MUCL49 410, R. clarus MUCL46 238, R. aggregatus MUCL49 408), were considered and their effects on the PMs and SMs of A. officinalis evaluated under controlled semi-hydroponic cultivation conditions. An untargeted metabolomic analysis was performed using UHPLC-HRMS followed by a multivariate data analysis. Forty-two compounds were reported to be highly modulated in relation to the different AMF associations. Among them, six new SMs were tentatively identified including two acetyl- and four malonyl- phenylpropanoid and saponin derivatives, all presenting a common substitution at position C-6′′ of the glycosidic moiety. In addition, an enhanced accumulation of PMs and SMs was observed for R. irregularis and R. intraradices, showing a stronger effect on Anchusa officinalis metabolome compared to R. clarus and R. aggregatus. Therefore, our data suggest that different AMF species belonging to the same genus specifically modulate the metabolome of A. officinalis. This observation can contribute to the selection of the most adequate AMF species for the production of targeted compounds.



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Artikel online veröffentlicht:
12. Dezember 2022

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  • Reference

  • 1 Cartabia A, Tsiokanos E, Tsafantakis N. et al. The Arbuscular Mycorrhizal Fungus Rhizophagus irregularis MUCL41833 Modulates Metabolites Production of Anchusa officinalis L. Under Semi-Hydroponic Cultivation. Front Plant Sci 2021; 12: 1766
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