Metabolomic analysis of Aspergillus tubingensis (IBT23488)

The black aspergilli (Aspergillus section Nigri) are an important group of species in food, medical mycology and biotechnology. In biotechnology, they are used in many processes such as for the production of biomass, organic acids, enzymes and isolation of secondary metabolites. A. tubingensis strains have been reported to be prolific producers for many classes of natural products like alkaloids, anthraquinones, terpenoids and γ-naphthopyrones. Moreover, some of the above-mentioned secondary metabolites shows significant biological activity and also represent novel structure, such as antiviral tubingensins. In present study, A. tubingensis IBT23488, forming sclerotia, was fermented in YES media and LC-MS analysis shows the presence of aurasperone C and E, malformin A1, tubingensin A and B, alfavinine as well as some new indole diterpenes. Till now, purification of the crude ethyl acetate extract of A. tubingensis led to the isolation of a known compound nigragilin. Isolation of new indole terpenoids is on-going. Furthermore, while co-culture of the fungus A. tubingensis in various media (OAT, PDA and CYA) with bacteria of genus Streptomyces indicate the suppression of the production of some secondary metabolites, it seems that amphotericin-producing Streptomyces induce the production of indole terpenes, the same way as amphotericin B in preliminary experiments.

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Artikel online veröffentlicht:
12. Dezember 2022