Short Lecture “Cell cultures of Dracocephalum ruyschiana and Juniperus communis – valuable sources of skin protecting and regenerating compounds”

Plant cell cultivation is a promising technology for sustainable production of plant secondary metabolites. Extracts containing such compounds are particularly interesting for cosmetics industry. Over the last decades there has been significant increase in use of plant cell culture derived ingredients in cosmetics and the demand continues to grow. Aim of this study was to evaluate various extraction processes to leverage the full potential of Dracocephalum ruyschiana and Juniperus communis cell cultures. Various solvents (water, ethanol, glycerin, propandiol, pentylene glycol) as well as supercritical fluid extraction was performed to produce extracts. Moreover, remaining by-products after the primary extraction underwent second extraction step to explore the potential of the biorefinery approach for production of various ingredients form the same culture. Chromatography analyses showed the presence of phenolic compounds and flavonoids in D. ruyschiana cell biomass extract, with rosmarinic acid, and caffeic acid being the dominating ones. J. communis cell culture extracts were rich in procyanidins. Remaining biomass after the primary extraction proved to be a valuable source of amino acids, polysaccharides, glycoproteins. Chemical composition varied depending on the cell cultivation conditions and extraction protocols. In vitro testing showed high safety of the extracts in cytotoxicity and phototoxicity assays. Stimulatory effect on proliferation of skin cells was observed. Quantification of reactive oxygen species by flow cytometry showed high antioxidative activity. Chemical composition and bioactivity data substantiates the application of plant cell cultivation approach for production of D. ruyschiana and J. communis derived cosmetic ingredients.

Acknowledgments

The work has been supported by ERDF project No. 1.1.1.1/19/A/075

Publikationsverlauf

Artikel online veröffentlicht:
12. Dezember 2022