Planta Med 2022; 88(15): 1412
DOI: 10.1055/s-0042-1758939
Short Lectures B: Biotechnology – Bioengineering

Short Lecture “In vitro propagation of the medicinal halophyte Polygonum maritimum L. and phenolic composition of produced plants”

L Custódio
1   Centre of Marine Sciences, University of Algarve, Faculty of Sciences and Technology, Ed. 7, Campus of Gambelas, 8005-139 Faro, Portugal
,
S Ślusarczyk
2   Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, Wroclaw, Poland
,
A Matkowski
2   Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, Wroclaw, Poland
,
E Fernandes
1   Centre of Marine Sciences, University of Algarve, Faculty of Sciences and Technology, Ed. 7, Campus of Gambelas, 8005-139 Faro, Portugal
,
V Castañeda-Loaiza
1   Centre of Marine Sciences, University of Algarve, Faculty of Sciences and Technology, Ed. 7, Campus of Gambelas, 8005-139 Faro, Portugal
,
C Pereira
1   Centre of Marine Sciences, University of Algarve, Faculty of Sciences and Technology, Ed. 7, Campus of Gambelas, 8005-139 Faro, Portugal
,
M Rodrigues∗
1   Centre of Marine Sciences, University of Algarve, Faculty of Sciences and Technology, Ed. 7, Campus of Gambelas, 8005-139 Faro, Portugal
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Polygonum maritimum L. is a medicinal halophyte with anti-inflammatory properties, ascribed to flavonoids, such as myricetin and quercetin glycosides [1], [2], [3]. Therefore, this study aimed at establishing a micropropagation procedure of P. maritmum for potential commercial cultivation, by enhancing shoot multiplication, rooting and acclimatization procedures, followed by the assessment of the phenolic profile of produced plants. The combination of 3 mg/L BA + 0.1 mg/L IAA induced the maximum shoot formation (10.3), which significantly increased in the second cycle (18.3). The best rooting capacity was observed on shoots derived from the control medium (100%), followed by 2 mg/L KIN (97%) and 3 mg/L BA + 0.1 mg/L IAA (90%), however the shoot number in the end of rooting phase was higher on shoots derived from 3 mg/L BA + 0.1 mg/L IAA (6.16). The plant growth regulators used in the multiplication phase influenced survival in the acclimatization process, and plants derived from the control medium had the highest survival percentage (63.1%). Acetone extracts made from aerial organs of micropropagated P. maritimum showed a predominance of the flavonoid myricetin-3-O-rhamnoside (8.135 mg/g), ascribed with anti-inflammatory ability. Overall, P. maritimum was successfully micropropagated showing their potential as a medicinal crop for extraction of anti-inflammatory molecules.

Funding

This research was funded by FCT and Portuguese National Budget (UIDB/04 326/2020 and UID/DTP/04 138/2020), and the HaloFarMs project (PRIMA Programme, supported by European Union and FCT. LC was supported by FCT Scientific Employment Stimulus (CEECIND/00 425/2017). VCL and EF acknowledges FCT for PhD grants (2020. 04 541.BD and UI/BD/151 301/2121, respectively).


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Artikel online veröffentlicht:
12. Dezember 2022

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  • References

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