CC BY-NC-ND 4.0 · J Lab Physicians 2023; 15(02): 311-315
DOI: 10.1055/s-0042-1758663
Case Report

Anti-F-Actin Antibody Positivity on Indirect Immunofluorescence Assay Following Chinese and Alternative Medicine Therapy: A Case Report

1   Department of Biochemistry, All India Institute of Medical Sciences (AIIMS) Patna, Patna, Bihar, India
1   Department of Biochemistry, All India Institute of Medical Sciences (AIIMS) Patna, Patna, Bihar, India
Soma Dey
1   Department of Biochemistry, All India Institute of Medical Sciences (AIIMS) Patna, Patna, Bihar, India
Ramesh Kumar
2   Department of Gastroenterology, All India Institute of Medical Sciences (AIIMS) Patna, Patna, Bihar, India
› Author Affiliations


Immunofluorescence on human epithelial type 2 cells is the standard screening assay for the detection of antinuclear antibodies (ANA). Cytoplasmic speckled patterns are a common finding. However, the less commonly reported ones include the cytoplasmic fibrillar patterns on indirect immunofluorescence technique (IIFT). The cytoplasmic fibrillar patterns include the cytoplasmic linear (AC-15), cytoplasmic filamentous (AC-16), and cytoplasmic segmental (AC-17). We report a case of cytoplasmic linear (F-actin) detected through IIFT during ANA screening in a 77-year-old man and later reconfirmed on liver mosaic biochip through IIFT on vascular smooth muscle substrate (VSM-47) without features suggestive of anti-smooth muscle antibody involvement post-complementary and alternative medicine therapy initiation.

Publication History

Article published online:
05 December 2022

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