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CC BY-NC-ND 4.0 · J Lab Physicians 2022; 14(04): 394-397
DOI: 10.1055/s-0042-1757230
Original Article

Correlation of Thrombosis and Clinicohematological Parameters with JAK2V617F Mutation in Philadelphia-Negative CMPNs: A Study from India

Kanwaljeet Singh
1   Department of Laboratory Sciences and Molecular Medicine, Army Hospital (R&R), Delhi Cantt, India
,
Pradeep V.
1   Department of Laboratory Sciences and Molecular Medicine, Army Hospital (R&R), Delhi Cantt, India
,
Ankur Ahuja
1   Department of Laboratory Sciences and Molecular Medicine, Army Hospital (R&R), Delhi Cantt, India
,
Venkatesan Somasundarum
1   Department of Laboratory Sciences and Molecular Medicine, Army Hospital (R&R), Delhi Cantt, India
,
Kundan Mishra
1   Department of Laboratory Sciences and Molecular Medicine, Army Hospital (R&R), Delhi Cantt, India
,
Tathagat Chatterjee
1   Department of Laboratory Sciences and Molecular Medicine, Army Hospital (R&R), Delhi Cantt, India
› Institutsangaben Funding None.
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Abstract

Objective Philadelphia-negative chronic myeloproliferative neoplasms (CMPNs), which include polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF), are characterized by the presence of JAK2V617F (exon 14) mutation, and this occurs in 90 to 95% cases of PV and 50 to 60% cases of ET and PMF. Still, this is a matter of debate regarding the correlation of this mutation with thrombosis and clinicohematological parameters in CMPNs. So, we conducted this study to ascertain the association of JAK2V617F mutation with thrombotic complications and clinicohematological parameters of these patients.

Materials and Methods This prospective and retrospective study was conducted during 2018 to 2019 at the Department of Laboratory Sciences and Molecular Medicine of a tertiary care hospital, and 160 CMPN patients were enrolled. Complete hemogram was done and DNA was extracted, followed by real-time qualitative polymerase chain reaction to check for JAK2V617F mutation. This mutation was then correlated with complications, mainly thrombosis, hematological parameters, and clinical parameters such as age and splenomegaly.

Results Among 160 CMPN patients, 60 were females and 100 were males, with male to female ratio of 1:0.6, and age range of 27 to 85 years. Total 91 (56.9%) patients were JAK2V617F positive and the remaining 69 (43.1%) were negative for this mutation. We observed statistically significant correlation of leukocyte count, splenomegaly, and thrombosis in JAK2V617F-mutated patients as compared to unmutated patients.

Conclusion This study emphasizes the importance of JAK2V617F mutation in CMPNs and stresses on its association with clinical, hematological phenotype, and thrombotic complications, which may open new horizons in prognostication and management protocol.

Keywords

JAK2V617F mutation - Philadelphia-negative CMPNs - thrombosis


Publikationsverlauf

Artikel online veröffentlicht:
20. Oktober 2022

© 2022. The Indian Association of Laboratory Physicians. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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