Interzellulärer Transfer von Glykosylphosphatidylinositol-verankerten Proteinen zwischen menschlichen Adipozyten oder zwischen Blutzellen und Adipozyten reguliert die basale Lipidsynthese (#28)

Einleitung Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are anchored at the outer leaflet of plasma membranes (PM) by a carboxy-terminal GPI glycolipid, and fulfill multiple functions at the cell surface. GPI-APs with the complete GPI anchor can be transferred between donor and acceptor cells, the physiological role of which remains unclear.

Methoden Differentiated human adipocytes with reduced expression of GPI-APs at PM were used as acceptor cells and incubated with GPI-APs, prepared from primary rat adipocytes and embedded in micelle-like complexes, or with erythroleukemia (EL) cells and human adipocytes with normal expression of GPI-APs as donor cells in transwell co-cultures.

Ergebnisse Upregulation of GPI-APs detected at PM of adipocytes and lipid synthesis was observed which followed similar time courses and was abrogated by serum, albumin and depletion of total, but not selected GPI-APs. Adipocytes of low size were shown to act as lipid-synthesizing acceptor cells with higher efficacy compared to large lipid-filled adipocytes which are more productive as donor cells for GPI-APs.

Schlussfolgerung Full-length GPI-APs are transferred between blood cells and adipocytes resulting in stimulation of lipid synthesis under control of serum factors. This argues for the (patho)physiological relevance of intercellular transfer of GPI-APs in (dys)regulation of lipid metabolism, and raises the possibility for use of transfer of GPI-APs as target for the therapy of obesity.

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Artikel online veröffentlicht:
11. Oktober 2022