Effects of Chrysin on Oral Squamous Cell Carcinoma In Vitro

Duangchewan Puengsurin; Supranee Buranapraditkun; Chayanee Leewansangtong; Nitchakarn Taechaaukarakul; Proud Songsivilai; Rudee Surarit; Nakarin Kitkumthorn

doi:10.1055/s-0042-1755624

European Journal of Dentistry, Table of Contents

CC BY 4.0 · Eur J Dent 2023; 17(03): 797-803
DOI: 10.1055/s-0042-1755624

Original Article

Effects of Chrysin on Oral Squamous Cell Carcinoma In Vitro

Authors

Duangchewan Puengsurin

¹Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
Supranee Buranapraditkun

²Division of Allergy and Clinical Immunology, Department of Medicine, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Thai Red Cross Society, Bangkok 10330, Thailand

³Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center-Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand

⁴Thai Pediatric Gastroenterology, Hepatology and Immunology (TPGHAI) Research Unit, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, The Thai Red Cross Society, Bangkok 10330, Thailand
Chayanee Leewansangtong

¹Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
Nitchakarn Taechaaukarakul

¹Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
Proud Songsivilai

¹Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
Rudee Surarit

¹Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand
Nakarin Kitkumthorn

¹Department of Oral Biology, Faculty of Dentistry, Mahidol University, Nakhon Pathom, Thailand

Abstract

Objective Chrysin is a hydroxylated flavonoid derived from “propolis or bee glue,” a natural product. Previous research on chrysin's biological functions, including anticancer activity, had been reported. However, chrysin's effect on oral squamous cell carcinoma (OSCC) is still scarce. This article aimed to test the cytotoxicity, antiproliferative, antimigration, anti-invasion, and apoptotic effects of purified chrysin in two OSCC cell lines, HSC4 and SCC25.

Materials and Methods The malignant phenotype was assessed using cell proliferation, wound healing, and transwell assays. Cell apoptosis was determined using flow cytometry. The positive control was OSCC cells treated with cisplatin, and the negative control was OSCC cells incubated with 0.1% dimethyl sulfoxide.

Results Chrysin at concentrations of 100 and 200 µM could inhibit OSCC cell proliferation, migration, and invasion, as well as enhance cell apoptosis, particularly in the early stages of apoptosis.

Conclusion In OSCC cell lines, chrysin has been demonstrated to be an effective antioncogenic agent. Additional research is required to confirm the results. Chrysin should be suggested as a possible alternative therapeutic application for OSCC.

Keywords

chrysin - oral squamous cell carcinoma - proliferation - migration - invasion - apoptosis

Full Text

References

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