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CC BY-NC-ND 4.0 · Journal of Coloproctology 2022; 42(03): 238-244
DOI: 10.1055/s-0042-1755346
Original Article

Campsiandra laurifolia Reduces Oxidative Stress and Inflammation in Rats with Ulcerative Colitis

Sandielly Rebeca Benitez da Fonseca 
1   Post-graduation Program in Medical Sciences, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
2   Experimental Laboratory of Pulmonary Sciences and Inflammation, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil
,
Lucas Petitemberte de Souza 
3   Post-graduation Program in Biotechnology, Universidade Federal de Pelotas (UFPEL), Pelotas, RS, Brazil
,
Henrique Sarubbi Fillmann 
4   School of Medicine, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil
,
Renata Minuzzo Hartmann 
2   Experimental Laboratory of Pulmonary Sciences and Inflammation, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil
,
Josieli Raskopf Colares 
2   Experimental Laboratory of Pulmonary Sciences and Inflammation, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil
,
Elizângela Gonçalves Schemitt 
2   Experimental Laboratory of Pulmonary Sciences and Inflammation, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil
,
Marilda da Silva Brasil 
2   Experimental Laboratory of Pulmonary Sciences and Inflammation, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil
5   Post-graduation Program in Biological Sciences - Fisiology, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
,
Edna Sayuri Suyenaga 
6   Health School, Universidade do Vale do Rio dos Sinos (UNISINOS), São Leopoldo, RS, Brazil
,
Norma Anair Possa Marroni 
1   Post-graduation Program in Medical Sciences, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
2   Experimental Laboratory of Pulmonary Sciences and Inflammation, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil
5   Post-graduation Program in Biological Sciences - Fisiology, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
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Abstract

Ulcerative colitis (UC) affects the mucosa and submucosa of the large intestine. One of the mechanisms involved in its etiology is oxidative stress (OS), directly involved in the inflammatory process characteristic of UC. The Campsiandra laurifolia, known as acapurana, was described as possessing antioxidant properties. We used 24 male Wistar rats, divided into control (CO), control + acapurana (CO + A), colitis (CL), and colitis + acapurana (CL + A) groups. This study performed histological analysis, measuring anal sphincter pressure (ASP) and lipoperoxidation (LPO). The activity of the antioxidant enzyme superoxide dismutase (SOD) and glutathione (GSH) levels were evaluated. The expression of the nuclear factor kappa B (NFκB) and inducible nitric oxide synthase (iNOS) was analyzed by immunohistochemistry. The statistical analysis used was the one-way analysis of variance (ANOVA), followed by the Student-Newman-Keuls test; values were expressed as mean ± standard error, and the significance level was p < 0.05. In the animals of the CL group, we observed the destruction of the crypts and the presence of mucosal ulcers, edema, and submucosal inflammatory infiltrate, as well as increased damage to the intestinal mucosa, reduced ASP, increased LPO and SOD activity, reduced GSH levels, and increased expression of NFκB and iNOS. The administration of C. laurifolia in the CL + A group was shown to cause regeneration of crypts, reduction of inflammatory infiltrate, reduction of damage to the intestinal mucosa, increase in ASP, and reduction in LPO with the restoration of SOD activity and GSH levels. The immunohistochemistry of NFκB and iNOS was significantly reduced. Therefore, the C. laurifolia aqueous extract appears to exert an antioxidant and anti-inflammatory effect in rats with AA-induced colitis.

Keywords

ulcerative colitis - antioxidant - anti-inflammatory - campsiandra laurifolia

Support Sources

CAPES; CNPq; FIPE-HCPA.




Publication History

Received: 15 April 2022

Accepted: 06 July 2022

Article published online:
20 September 2022

© 2022. Sociedade Brasileira de Coloproctologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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