The Effect of Probiotic Lactobacillus acidophilus and Ethanolic Propolis Compound toward Nucleic Acid Deposition in the Extracellular Polymeric Substance of Root Canal Bacteria

Arya Adiningrat; Rifkhi A. Kusnadi; Asyam S. Allam; Erma Sofiani; Ikhsan Maulana; Hiromichi Yumoto

doi:10.1055/s-0042-1750771

European Journal of Dentistry, Table of Contents

CC BY 4.0 · Eur J Dent 2023; 17(02): 418-423
DOI: 10.1055/s-0042-1750771

Original Article

The Effect of Probiotic Lactobacillus acidophilus and Ethanolic Propolis Compound toward Nucleic Acid Deposition in the Extracellular Polymeric Substance of Root Canal Bacteria

Arya Adiningrat

¹Department of Oral Biology and Biomedical Sciences, School of Dentistry, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia

²Molecular Medicine and Therapy Laboratory of Research Division, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Indonesia

,

Rifkhi A. Kusnadi

³Clinical Program of School of Dentistry, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Indonesia

,

Asyam S. Allam

³Clinical Program of School of Dentistry, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Indonesia

,

Erma Sofiani

⁴Department of Endodontology, School of Dentistry, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia

,

Ikhsan Maulana

²Molecular Medicine and Therapy Laboratory of Research Division, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Indonesia

,

Hiromichi Yumoto

⁵Department of Periodontology and Endodontology, Tokushima University Graduate School of Biomedical Sciences, Japan

› Author Affiliations

Abstract

Objective This study aimed to evaluate the effects of Apis trigona ethanolic propolis and probiotic bacteria Lactobacillus acidophilus on the nucleic acid concentration in the extracellular polymeric substances (EPS) derived from biofilm of root canal bacteria.

Materials and Methods Clinical bacteria of the root canal were cultured with ethanolic extract of propolis (EEP; 10 or 0.1%) and L. acidophilus. After the formation of biofilm was observed in the monolayer bacterial culture under several conditions, the enzymatic treatment and nucleic acid quantification were sequentially performed.

Statistical Analysis Independent t-test and Mann–Whitney were performed following data normality to analyze the significant differences of the treatment effect on the nucleic acid concentration in EPS from the isolated biofilm.

Results The results showed that the nucleic acid concentration in EPS biofilm were not increased by coculture with L. acidophilus as probiotics. However, the treatment with 10% EEP could significantly increase nucleic acid concentration.

Conclusion This study suggested that the biosurfactants from probiotic bacteria L. acidophilus might be a promising candidate for endodontic treatment, arguably better than EEP in inhibiting biofilm maturation and complexity.

Keywords

ethanolic extract of propolis - extracellular polymeric substance - Lactobacillus acidophilus - extracellular nucleic acid - probiotics bacteria - root canal biofilm

Full Text

References

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