Zeitschrift für Phytotherapie 2022; 43(S 01): S44-S45
DOI: 10.1055/s-0042-1749284
Abstracts Poster | Phytotherapie 2022 – innovativ

Gene expression analyses support multi-target mode of action of black cohosh (Cimicifuga racemosa) against menopausal complaints

P Stute
1   Department of Obstetrics and Gynecology, University of Bern, Switzerland
,
S Ehrentraut
2   arrows biomedical Deutschland GmbH, Münster, Germany
,
H Henneicke-von Zepelin
3   Department of Regulatory Affairs, Schaper & Brümmer GmbH & Co. KG, Salzgitter, Germany
,
P Nicken
3   Department of Regulatory Affairs, Schaper & Brümmer GmbH & Co. KG, Salzgitter, Germany
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Introduction More in-depth evidence for the mode of action on how cimicifuga alleviates menopausal complaints is consistently emerging.

Aim To assess gene expression profiles within the hippocampus and hypothalamus of ovariectomized (OVX) rats with or without treatment with an isopropanolic extract of the rhizomes of Cimicifuga racemosa (iCR) in comparison to intact rats.

Method Exploratory study in hippocampal and hypothalamic tissue from 20 female Sprague Dawley rats. Group 1 (PRAE) presents hippocampal (n = 4) and hypothalamic (n = 4) tissues of rats without OVX. Group 2 (OVX) presents hippocampal (n = 4) and hypothalamic (n = 3) tissues three months after bilateral OVX. Group 3 (OVX+iCR) presents hippocampal (n = 2) and hypothalamic (n = 2) tissues of rats after their treatment with iCR for three months after OVX. Gene expression profiles in brain tissue were analyzed by RNA-microarray-analysis and subsequent verification by qPCR.

Results Overall, 4812 genes were differentially regulated when comparing the 3 groups in hippocampus and hypothalamus. iCR compensated the effects of ovariectomy in 518 genes. In particular, this compensatory effect was most prominent in the hippocampus involving genes like Gal, Calca, Hcrt, Avpr1a and Pnoc, which are related to blood pressure regulation, metabolism, hormonal regulation, homeostasis, learning and cognition, mood regulation, neuroendocrine modulation, thermoregulation, regulation of sleep and arousal. In the hypothalamus, iCR compensated the effect of ovariectomy at Tac3, Oprm1, but not at Kiss1. These genes are involved in the pathophysiology of hot flashes.

Conclusion Our findings support the evidence on the mode of action of iCR in alleviating menopausal complaints on a tissue-specific brain gene expression level. Beyond this, the impact of iCR on gene expression associated with cognition and nociception deserves further investigation [1].



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Artikel online veröffentlicht:
13. Juni 2022

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