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CC BY-NC-ND 4.0 · J Lab Physicians 2022; 14(04): 449-455
DOI: 10.1055/s-0042-1748826
Original Article

Species Distribution and Antifungal Susceptibility Profile of Dermatophytes from a Tertiary Care Centre in North India

Mani Bhushan Kumar
1   Department of Microbiology, Government Medical College Hospital, Chandigarh, India
,
Neelam Gulati 
1   Department of Microbiology, Government Medical College Hospital, Chandigarh, India
,
Jagdish Chander
1   Department of Microbiology, Government Medical College Hospital, Chandigarh, India
,
Nidhi Singla
1   Department of Microbiology, Government Medical College Hospital, Chandigarh, India
,
Mala Bhalla
2   Department of Dermatology, Venereology and Leprosy, Government Medical College Hospital, Chandigarh, India
,
Charu Nayyar
3   Department of Microbiology, Medanta Hospital, Sriganganagar, Rajasthan, India
,
Swati Sharma
1   Department of Microbiology, Government Medical College Hospital, Chandigarh, India
,
Manharpreet Kaur
1   Department of Microbiology, Government Medical College Hospital, Chandigarh, India
› Author Affiliations Funding None.
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Abstract

Objective Dermatophytoses, one of the most ancient diseases, is becoming a menace in recent times. This has made the knowledge of antifungal susceptibility a priority in today's times.

Material and Methods This is a prospective study conducted over 18 months including all dermatophytes isolated during the period. Dermatophytes were identified by routine phenotypic methods. Antifungal susceptibility testing was performed for griseofulvin, terbinafine, and itraconazole as per the Clinical Laboratory Standard Institute M38 A2, and minimum inhibitory concentrations (MICs) were read after 5 days.

Results Patient details and associated risk factors were recorded. Fixed dose combinations with steroids were associated with 79.3% (46 out of 58) of patients with dermatophytosis of skin. Among the 72 dermatophytes isolated during the study period, 58 (80.5%) were isolated from skin scrapings and 14 (19.4%) from nail samples. Tinea corporis with cruris was the most common presentation. The most common dermatophyte isolated from skin scrapings was Trichophyton mentagrophytes complex (70.6%, 41 out of 58), while from nail samples it was Trichophyton rubrum complex (78.57%, 11 out of 14). Based on the MIC50 and MIC90 results, itraconazole showed the lowest MICs, followed by terbinafine and then griseofulvin.

Conclusion With the changing epidemiology of species distribution and antifungal resistance, there is a need for continuous surveillance of these parameters of dermatophytes.

Keywords

Dermatophytes - Trichophyton - antifungal susceptibility - Griseofulvin - Terbinafine

Authors' Contribution

JC and NG are responsible for conceptualization; MBK, SS, and MK for methodology; MBK and NG for formal analysis and investigation; NG, NS, and CN for writing—original draft preparation; JC and MB for writing—review and editing; and JC for supervision.


Ethical Approval

All procedures performed in the study were in accordance with the ethical standard of the institute research committee and with the 1964 Helsinki declaration.




Publication History

Article published online:
07 June 2022

© 2022. The Indian Association of Laboratory Physicians. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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