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CC BY 4.0 · Eur J Dent 2023; 17(03): 881-888
DOI: 10.1055/s-0042-1748529
Original Article

Gingival Mesenchymal Stem Cells Metabolite Decreasing TRAP, NFATc1, and Sclerostin Expression in LPS-Associated Inflammatory Osteolysis In Vivo

Alexander Patera Nugraha
1   Dental Regenerative Research Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
2   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Nastiti Faradilla Ramadhani
1   Dental Regenerative Research Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
3   Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Wibi Riawan
4   Department of Biomolecular Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
,
Igo Syaiful Ihsan
5   Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Surabaya, Indonesia
,
Diah Savitri Ernawati
6   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Rini Devijanti Ridwan
7   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ida Bagus Narmada
1   Dental Regenerative Research Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
2   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Tania Saskianti
8   Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Fianza Rezkita
9   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Andari Sarasati
9   Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Tengku Natasha Eleena Binti Tengku Ahmad Noor
10   Membership Faculty of Dental Surgery, Royal Collage of Surgery, Edinburgh University, UK
,
Bilqis Inayatillah
11   Department of Basic Medical of Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Albertus Putera Nugraha
12   Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Florentina Joestandari
13   Faculty of Dentistry, Institute of Health Bhakti Wiyata, Kediri, Indonesia
› Author Affiliations Funding This study was supported by Hibah Internal Penelitian Dosen Pemula (PDP) 2021 with appointment number 212/UN3/2021 Universitas Airlangga, Surabaya, East Java, Indonesia.
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Abstract

Objective Bone is a dynamic tissue that undergoes remodeling. During bone remodeling, there are transcription factors such as nuclear factor-activated T cells-1 (NFATc1), sclerostin, and tartrate-resistant acid phosphatase (TRAP) that are released for bone resorption. Metabolite from gingival mesenchymal stem cells (GMSCs) has the ability to activate proliferation, migration, immunomodulation, and tissue regeneration of bone cells and tissues. Furthermore, the aim of this study is to investigate the metabolite of GMSCs' effect on expression of NFATc1, TRAP, and sclerostin in calvaria bone resorption of Wistar rats.

Materials and Methods Twenty male healthy Wistar rats (Rattus norvegicus), 1 to 2 months old, 250 to 300 g body were divided into four groups, namely group 1 (G1): 100 µg phosphate-buffered saline day 1 to 7; group 2 (G2): 100 μg lipopolysaccharide (LPS) day 1 to 7; group 3 (G3): 100 μg LPS + 100 μg GMSCs metabolite day 1 to 7; and group 4 (G4): 100 μg GMSCs metabolite day 1 to 7. Escherichia coli LPS was used to induce inflammatory osteolysis on the calvaria with subcutaneous injection. GMSCs metabolite was collected after passage 4 to 5, then injected subcutaneously on the calvaria. All samples were sacrificed on the day 8 through cervical dislocation. The expression of TRAP, NFATc1, and sclerostin of osteoclast in the calvaria was observed with 1,000× magnification.

Statistical Analysis One-way analysis of variance and Tukey honest significant different were conducted to analyze differences between groups (p < 0.05).

Results The administration of GMSCs metabolite can significantly decrease TRAP, NFATc1, and sclerostin expression (p < 0.05) in LPS-associated inflammatory osteolysis calvaria in Wistar rats (R. norvegicus). There were significantly different TRAP, NFATc1, and sclerostin expressions between groups (p < 0.05).

Conclusion GMSCs metabolite decrease TRAP, NFATc1, and sclerostin expression in LPS-associated osteolysis calvaria in Wistar rats (R. norvegicus) as documented immunohistochemically.

Keywords

stem cells - lipopolysaccharide - medicine - infectious disease - dentistry


Publication History

Article published online:
21 June 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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