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CC BY 4.0 · Eur J Dent 2023; 17(04): 1037-1042
DOI: 10.1055/s-0042-1748528
Original Article

The Effect of Centella asiatica Methanolic Extract on Expression of IL-1β Proinflammatory Cytokines in Severe Early Childhood Caries

Muhammad Luthfi
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Fathillah Abdul Razak
2   Department of Oral and Craniofacial Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia
,
Devy Putri Kusumawardhani
3   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ayu Anggraini Broto Nagoro
3   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Naura Fadhila
3   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
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Abstract

Objective This article analyzes the role of C. asiatica extract in reducing the proinflammatory cytokine interleukin (IL)-1β produced by salivary neutrophils.

Material and Methods Selected kindergartens in the Surabaya area provided samples. The sample was initially checked for dental caries by measuring its def-t index, and then the participants who satisfied the requirements for severe caries with a def-t of greater than 6 were chosen. At the time of sampling, all of the individuals were between the ages of 4 and 6. The sampling was performed by researchers and certified persons using well-known methodologies. For 60 minutes before to sampling, respondents were not allowed to eat, drink, chew gum, or brush their teeth. For analysis, the samples were collected and then frozen at −80°C.

Results The administration of methanolic extract C. asiatica decreased the expression of the proinflammatory cytokine IL-1β on the surface of salivary neutrophils on S-ECC; The administration of C. asiatica methanol extract resulted in a decrease in the expression of the proinflammatory cytokine IL-1β on the surface of salivary neutrophils in S-ECC.

Conclusion C. asiatica extract has the effect of reducing the proinflammatory cytokine IL-1β produced by salivary neutrophils on S-ECC via inhibiting nuclear factor kappa B and mitogen–activated protein kinase signaling pathway activation and suggest that C. asiatica is a possible candidate for treating S-ECC.

Keywords

Centella asiatica - S-ECC - IL-1β - chronic diseases - medicine


Publication History

Article published online:
09 August 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Indrawati R. Aktivitas Enzim Dextranase Dan Sebaran Genotipe Streptococcus mutans Pender1ta Karies Dan Bebas Karies. Thesis Dissertation.. Published online 2006 . Available at: https://repository.unair.ac.id/31914/
    PubMedGoogle Scholar
  • 2 Filoche S, Wong L, Sissons CH. Oral biofilms: emerging concepts in microbial ecology. J Dent Res 2010; 89 (01) 8-18
    CrossrefPubMedGoogle Scholar
  • 3 Tinanoff N, Reisine S. Update on early childhood caries since the Surgeon General's Report. Acad Pediatr 2009; 9 (06) 396-403
    CrossrefPubMedGoogle Scholar
  • 4 Vollú AL, da Costa MDEPR, Maia LC, Fonseca-Gonçalves A. Evaluation of oral health-related quality of life to assess dental treatment in preschool children with early childhood caries: a preliminary study. J Clin Pediatr Dent 2018; 42 (01) 37-44
    CrossrefPubMedGoogle Scholar
  • 5 Kassebaum NJ, Smith AGC, Bernabé E. et al; GBD 2015 Oral Health Collaborators. Global, regional, and national prevalence, incidence, and disability-adjusted life years for oral conditions for 195 countries, 1990–2015: a systematic analysis for the global burden of diseases, injuries, and risk factors. J Dent Res 2017; 96 (04) 380-387
    CrossrefPubMedGoogle Scholar
  • 6 Kawashita Y, Kitamura M, Saito T. . Early childhood caries. Int J Dent 2011; 2011: 725320
    CrossrefPubMedGoogle Scholar
  • 7 Riskesdas.. Main Results of Riskesdas 2018. Ministry of Health Republic of Indonesia. 2018: 1-100
    PubMedGoogle Scholar
  • 8 Daly B, Batchelor P, Treasure E, Watt R. Essential dental public health. Oxford University Press; USA: 2nd Ed., 2018
    Google Scholar
  • 9 Elbim C, Katsikis PD, Estaquier J. Neutrophil apoptosis during viral infections. Open Virol J 2009; 3 (03) 52-59
    CrossrefPubMedGoogle Scholar
  • 10 Castro NJ, Patel R, Zhang LG. Design of a novel 3D printed bioactive nanocomposite scaffold for improved osteochondral regeneration. Cell Mol Bioeng 2015; 8 (03) 416-432
    CrossrefPubMedGoogle Scholar
  • 11 Mantovani A, Cassatella MA, Costantini C, Jaillon S. Neutrophils in the activation and regulation of innate and adaptive immunity. Nat Rev Immunol 2011; 11 (08) 519-531
    CrossrefPubMedGoogle Scholar
  • 12 Jamil SS, Nizami Q, Salam M. Centella asiatica (Linn.) Urban—a review. Nat Prod Radiance 2007; 6 (02) 158-170
    Google Scholar
  • 13 Liu CC, Zhang Y, Dai BL. et al. Chlorogenic acid prevents inflammatory responses in IL—1β—stimulated human SW—1353 chondrocytes, a model for osteoarthritis. Mol Med Rep 2017; 16 (02) 1369-1375
    CrossrefPubMedGoogle Scholar
  • 14 Jayathirtha MG, Mishra SH. Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica. Phytomedicine 2004; 11 (04) 361-365
    CrossrefPubMedGoogle Scholar
  • 15 Bent R, Moll L, Grabbe S, Bros M. Interleukin-1 beta—a friend or foe in malignancies?. Int J Mol Sci 2018; 19 (08) 2155
    CrossrefPubMedGoogle Scholar
  • 16 Luthfi M, , Yuliati, Wijayanti EH, Abdul Razak FB, Irmalia WR. The efficacy of okra fruit extract on the expression of transforming growth factor beta 1 in the tooth socket of diabetic Wistar rats. Dent Res J (Isfahan) 2021; 18: 91
    CrossrefPubMedGoogle Scholar
  • 17 Luthfi M, Kusumaningsih T. Salivary neutrophils isolation of severe early childhood caries patients with flow cytometry analysis using magnetic beads and CD177 marker. Dent J 2016; 49 (01) 33-37 (Majalah Kedokteran Gigi)
    CrossrefGoogle Scholar
  • 18 Vasilyev FF, Lopatnikova JA, Sennikov SV. Optimized flow cytometry protocol for analysis of surface expression of interleukin-1 receptor types I and II. Cytotechnology 2013; 65 (05) 795-802
    CrossrefPubMedGoogle Scholar
  • 19 Luthfi M, Setijanto D, Rahardjo MB. et al. Correlation between human neutrophil peptide 1-3 secretion and azurophilic granule (CD63) expression in early childhood caries. Dent Res J (Isfahan) 2019; 16 (02) 81-86
    CrossrefPubMedGoogle Scholar
  • 20 Takeuchi O, Akira S. Pattern recognition receptors and inflammation. Cell 2010; 140 (06) 805-820
    CrossrefPubMedGoogle Scholar
  • 21 Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1β generation. Clin Exp Immunol 2007; 147 (02) 227-235
    CrossrefPubMedGoogle Scholar
  • 22 Kaminska B. MAPK signalling pathways as molecular targets for anti-inflammatory therapy–from molecular mechanisms to therapeutic benefits. Biochim Biophys Acta 2005; 1754 (1-2): 253-262
    CrossrefPubMedGoogle Scholar
  • 23 Luthfi M, Retno I, Arundina I, Dachlan YP. Korelasi jumlah Streptococcus mutans (S. mutans) dan level ekspresi interlukin 8 (IL-8) pada severe early childhood caries. Maj Kedokt Gigi Indones 2015; 1 (02) 142-148
    CrossrefGoogle Scholar
  • 24 Gao R, Yang H, Jing S. et al. Protective effect of chlorogenic acid on lipopolysaccharide-induced inflammatory response in dairy mammary epithelial cells. Microb Pathog 2018; 124: 178-182
    CrossrefPubMedGoogle Scholar
  • 25 White SC, Pharoah MJ. . Oral Radiology Principles and Interpretation. 7th ed. Elsevier Ltd; 2014
  • 26 Wason JMS, Stecher L, Mander AP. Correcting for multiple-testing in multi-arm trials: is it necessary and is it done?. Trials 2014; 15 (01) 364
    CrossrefPubMedGoogle Scholar
  • 27 Rahman M, Hossain S, Rahaman A, Fatima N, Nahar T, Uddin B. Antioxidant activity of Centella asiatica (Linn.) Urban: impact of extraction solvent polarity. Biomolecules 2013; 1 (06) 27-32
    Google Scholar
  • 28 Idris FN, Nadzir MM. Antimicrobial activity of Centella asiatica on Aspergillus niger and Bacillus subtilis. Chem Eng Trans 2017; 56: 1381-1386
    Google Scholar
  • 29 Murray MT, Pizzorno JE. Textbook of Natural Medicine. 4th ed. Churchill Livingstone; 2012
    Google Scholar
  • 30 Overman A, Chuang CCMM, McIntosh M. Quercetin attenuates inflammation in human macrophages and adipocytes exposed to macrophage-conditioned media. Int J Obes 2011; 35 (09) 1165-1172
    CrossrefPubMedGoogle Scholar
  • 31 Osborn O, Olefsky JM. The cellular and signaling networks linking the immune system and metabolism in disease. Nat Med 2012; 18 (03) 363-374
    CrossrefPubMedGoogle Scholar
  • 32 Sato S, Norikura T, Mukai Y. Maternal quercetin intake during lactation attenuates renal inflammation and modulates autophagy flux in high-fructose-diet-fed female rat offspring exposed to maternal malnutrition. Food Funct 2019; 10 (08) 5018-5031
    CrossrefPubMedGoogle Scholar
  • 33 Suganami T, Tanimoto-Koyama K, Nishida J. et al. Role of the Toll-like receptor 4/NF-kappaB pathway in saturated fatty acid-induced inflammatory changes in the interaction between adipocytes and macrophages. Arterioscler Thromb Vasc Biol 2007; 27 (01) 84-91
    CrossrefPubMedGoogle Scholar