CC BY-NC-ND 4.0 · Laryngorhinootologie 2022; 101(S 02): S207
DOI: 10.1055/s-0042-1746691
Poster
Head-Neck-Oncology: HPV / Tumor marker

HPV DNA and RNA in sputum, smear and tissue from patients with non-neoplastic and malignant tonsilla palatina diseases – does tonsillectomy protect against cancer?

Elgar Susanne Quabius
1   Universitätsklinikum Schleswig-Holstein, Campus Kiel, Klinik für Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgie Kiel
,
Alessa Heinrichs
3   Universitätsmedizin Rostock, Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie "Otto Körner" Rostock
,
Dirk Haaser
1   Universitätsklinikum Schleswig-Holstein, Campus Kiel, Klinik für Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgie Kiel
,
André Kühnel
4   Asklepios Kliniken Hamburg Harburg, Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgie Hamburg
,
Florian Hoppe
5   Klinikum Oldenburg, Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgie Oldenburg
,
Robert Arndt Mlynski
3   Universitätsmedizin Rostock, Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Kopf- und Halschirurgie "Otto Körner" Rostock
,
Petra Ambrosch
1   Universitätsklinikum Schleswig-Holstein, Campus Kiel, Klinik für Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgie Kiel
,
Markus Hoffmann
1   Universitätsklinikum Schleswig-Holstein, Campus Kiel, Klinik für Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgie Kiel
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Previous studies describe a correlation between HPV-positivity and non-smoking in TSCC; p16INK4A-expression as surrogate-marker for HPV-DNA/RNA-positivity is discussed controversial. Here, these parameters are assessed in a prospective, consecutive setting. HPV-status of sputum and tonsillar swabs is analyzed to determine their validity as surrogate-marker for tissue-HPV-status.

TSCC- (n=52) and non-neoplastic tonsillar tissues (n=163) were analyzed. HPV-DNA- and HPV-RNA-status of total sputum, cellular fraction and supernatants, tonsillar swabs and tonsillar tissue was determined by (RT)-PCR. Immunohistochemistry determined p16INK4A-expression.

23/163 (14.2%) non-neoplastic tonsils were HPV DNA+. Five patients (3 HPV16; 2 HPV11) had active infections (RNA+), in their tonsils, sputum and swabs. Further 140/163 (85.9%) of these patients were either DNA+ or DNA+ in all samples. 21/52 (40.4%) TSCC were HPV DNA+: 14 HPV16 (13 RNA+); 7 HPV18 (4 RNA+). 40/52 (76.9%) TSCC patients showed congruent results in all biomaterials. In all biomaterials from a single patient the same HPV type was found. p16INK4A expression alone would have misclassified the HPV-status of 14/52 (26.2%) TSCCs. A significant correlation between non-smoking and HPV DNA+ was found.

This prospective study confirms TSCC-results regarding the discrepancy between HPV-status and p16INK4A-expression and the significant correlation between non-smoking and HPV-DNA-positivity. Sputum and/or swabs do also not consistently match tissue-results. This might have (detrimental) consequences if sputum and/or swabs were used to conclude on tissue-HPV-status. The five patients with active HPV-infections in non-neoplastic tonsils were possibly due to tonsillectomy spared from developing a TSCC.

Deutsche Krebshilfe (Fördernummer: 111777)



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Artikel online veröffentlicht:
24. Mai 2022

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