Download PDF
CC BY 4.0 · Eur J Dent 2023; 17(02): 450-455
DOI: 10.1055/s-0042-1745772
Original Article

Osteoblast Viability of Liquid Smoke Rice Hull and Nanoparticles Form as Periodontitis Treatment

Ira Arundina
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Indeswati Diyatri
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Wisnu S. Juliastuti
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Theresia I. Budhy
2   Department of Oral Pathology and Maxillofacial, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Meircurius D. C. Surboyo 
3   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Benni Iskandar 
4   School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan
5   Sekolah Tinggi Ilmu Farmasi, Pekanbaru, Riau, Indonesia
,
Sheryn M. Ramaniasari
6   Bachelor Dental Science Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Azzahra S. A. Moelyanto
6   Bachelor Dental Science Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Gustiadi Saputra
7   Magister of Immunology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
› Author Affiliations Funding This work was supported by Ministry of Higher Education Republic of Indonesia in the Schema Penelitian Dasar Unggulan Perguruan Tinggi (PDUPT) 2021 with grant number: 311/UN3.15/PT/2021.
› Further Information
   Permissions and Reprints

Abstract

Objective Rice husk liquid smoke nanoparticles have the potential to be developed as a drug because they have anti-inflammatory effects that can modulate the process of osteoblast stimulation through osteoblast stimulation by thorough small size and enter cells easily. The osteoblast is the key of alveolar regeneration in periodontitis treatment. This present study analyzed the differences of liquid smoke rice husk and nanoparticles of liquid smoke rice husk on osteoblast viability as periodontitis treatment

Materials and Methods The liquid smoke rice husk was obtained from the pyrolysis process. The nanoparticles were made with chitosan, maltodextrin, and difference of concentration of liquid smoke rice husk (such as 1, 2.5, 5, 7.5, 10, 12.5, 15, and 17.5%). The viability of osteoblast was analyzed by 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.

Statistical Analysis The data were analyzed using independent t-test to analyze the differences between liquid smoke rice husk and nanoparticles of liquid smoke rice husk, the significant was set a p<0.05.

Result The nanoparticles of liquid smoke rice husk showed higher osteoblast viability compared liquid smoke rice husk. The nanoparticles' concentration of 5, 7.5, and 10% showed higher osteoblast viability compared liquid smoke rice husk (p = 0.002, 0.000, and 0.001, respectively).

Conclusion The nanoparticles of liquid smoke rice husk showed higher viability of osteoblast. This confirmed that the nanoparticles were able to reduce the toxicity in the higher concentration of liquid smoke of rice husk.

Keywords

nanoparticles - liquid smoke - rice husk - osteoblast and human and health

Ethical Approval

Ethic al approval was obtained from the Ethical Clearance of Health Experiment Committee, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia, under registered number: 414/HRECC. FODM/VII/2021.




Publication History

Article published online:
08 July 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India

 

  • References

  • 1 Arundina I, Diyatri I, Surboyo MDC, Monica E, Afanda NM. Growth factor stimulation for the healing of traumatic ulcers with liquid rice hull smoke. J Taibah Univ Med Sci 2021; 16 (03) 431-439
    PubMedGoogle Scholar
  • 2 Farida C, Purnawati RD, Wijayahadi N. Pengaruh Pemberian Asap Cair Dosis Bertingkat Terhadap Penyembuhan Luka Bakar Derajat Dua Dangkal Pada Kelinci (Oryctolagus Cuniculus). Diponegoro Medical Journal 2019; 8 (01) 436-445
    Google Scholar
  • 3 Desvita H, Faisal M. Suhendrayatna. Preservation of meatballs with edible coating of chitosan dissolved in rice hull-based liquid smoke. Heliyon 2020; 6 (10) e05228
    CrossrefPubMedGoogle Scholar
  • 4 Kim SP, Yang JY, Kang MY, Park JC, Nam SH, Friedman M. Composition of liquid rice hull smoke and anti-inflammatory effects in mice. J Agric Food Chem 2011; 59 (09) 4570-4581
    CrossrefPubMedGoogle Scholar
  • 5 Leon L, Chung EJ, Rinaldi C. A brief history of nanotechnology and introduction to nanoparticles for biomedical applications. In: Chung EJ, Leon L, Rinaldi C. eds. Nanoparticles for Biomedical Applications: Fundamental Concepts, Biological Interactions and Clinical Applications. 1st ed.. Amsterdam, the Netherlands: Elsevier Inc.; 2019: 1-4
    Google Scholar
  • 6 Tarudji AW, Kievit FM. Active targeting and transport. In: Chung EJ, Leon L, Rinaldi C. eds. Nanoparticles for Biomedical Applications: Fundamental Concepts, Biological Interactions and Clinical Applications. 1st ed.. Amsterdam, the Netherlands: Elsevier Inc.; 2019: 19-36
    Google Scholar
  • 7 Irianto HE. Proses dan aplikasi nanopartikel kitosan. Squalen. 2011; 6 (01) 1-8
    Google Scholar
  • 8 Bueno J. Nanotoxicity: the impact of increasing drug bioavailability. the impact of increasing drug bioavailability. In: Shegokar R. ed. Nanopharmaceuticals: Volume 1: Expectations and Realities of Multifunctional Drug Delivery Systems. Amsterdam, the Netherlands: Elsevier; 2020: 121-133
    Google Scholar
  • 9 Pinheiro AC, Bourbon AI, Cerqueira MA. et al. Chitosan/fucoidan multilayer nanocapsules as a vehicle for controlled release of bioactive compounds. Carbohydr Polym 2015; 115: 1-9
    CrossrefPubMedGoogle Scholar
  • 10 Rachmawati H, Larasati A, Adi AC, Shegokar R. Role of nanocarriers and their surface modification in targeting delivery of bioactive compounds. In: Shegokar R. ed. Nanopharmaceuticals: Volume 1: Expectations and Realities of Multifunctional Drug Delivery Systems. Amsterdam, the Netherlands: Elsevier; 2020: 17-43
    Google Scholar
  • 11 Kansara K, Kumar A. In vitro methods to assess the cellular toxicity of nanoparticles. In: Rajendran S, Mukherjee A, Nguyen TA, Godugu C, Shukla RK. (Eds) Micro and nano technologies, nanotoxicity. New York NY, USA: Elsevier Publishers; 2020: 21-40
    Google Scholar
  • 12 Kamiloglu S, Sari G, Ozdal T, Capanoglu E. Guidelines for cell viability assays. Food Frontiers. 2020; 1 (03) 332-349
    CrossrefGoogle Scholar
  • 13 Arundina I, Diyatri I, Dwi M, Surboyo C. The component analysis of liquid smoke from rice hulls and its toxicity test on baby hamster kidney cells. J Pharm Pharmacogn Res 2021; 9 (01) 78-87
    CrossrefGoogle Scholar
  • 14 Budhy TI, Arundina I, Surboyo MDC, Halimah AN. The effects of rice husk liquid smoke in porphyromonas gingivalis-induced periodontitis. Eur J Dent 2021; 15 (04) 653-659
    Article in Thieme ConnectPubMedGoogle Scholar
  • 15 Arundina IRA, Diyatri I, Surboyo MDC, Halimah AN, Chusnurrafi FI. The antibacterial effect of liquid smoke rice hull on porphyromonas gingivalis and its proliferative effects on osteoblast as periodontitis remedies: an invitro study. International Journal of Pharmaceutical Research. 2020; 12 (03) 3466-3471
    Google Scholar
  • 16 Arundina I, Tantiana T, Diyatri I, Surboyo MDC, Adityasari R. Acute toxicity of liquid smoke of rice hull (Oryza sativa) on mice (Mus musculus). Journal of International Dental and Medical Research. 2020; 13 (01) 91-96
    Google Scholar
  • 17 Saloko S, Darmadji P, Setiaji B, Pranoto Y. Structural analysis of spray-dried coconut shell liquid smoke powder. J Teknol Ind Pangan 2012; 23 (02) 173-178
    CrossrefGoogle Scholar
  • 18 Ganguly S, Ashley LA, Pendleton CM. et al. Characterization of osteoblastic properties of 7F2 and UMR-106 cultures after acclimation to reduced levels of fetal bovine serum. Can J Physiol Pharmacol 2008; 86 (07) 403-415
    CrossrefPubMedGoogle Scholar
  • 19 Risfaheri R, Hoerudin H, Syakir M. Utilization of rice husk for production of multifunctional liquid smoke. Journal of Advanced Agricultural Technologies. 2018; 5 (03) 192-197
    CrossrefGoogle Scholar
  • 20 Surboyo MDC, Arundina I, Rahayu RP, Mansur D, Bramantoro T. Potential of distilled liquid smoke derived from coconut (Cocos nucifera L) shell for traumatic ulcer healing in diabetic rats. Eur J Dent 2019; 13 (02) 271-279
    Article in Thieme ConnectPubMedGoogle Scholar
  • 21 Varkey A, Venugopal E, Sugumaran P. et al. Impact of silk fibroin-based scaffold structures on human osteoblast MG63 cell attachment and proliferation. Int J Nanomedicine 2015; 10 (October, suppl 1): 43-51
    PubMedGoogle Scholar
  • 22 Scozzafava A, Passaponti M, Supuran CT, Gülçin İ. Carbonic anhydrase inhibitors: guaiacol and catechol derivatives effectively inhibit certain human carbonic anhydrase isoenzymes (hCA I, II, IX and XII). J Enzyme Inhib Med Chem 2015; 30 (04) 586-591
    CrossrefPubMedGoogle Scholar
  • 23 Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Advances 2015; 5: 27986-28006
    CrossrefGoogle Scholar
  • 24 Arundina I, Diyatri I, Kusumaningsih T, Surboyo MDC, Monica E, Afanda NM. The role of rice hull liquid smoke in the traumatic ulcer healing. Eur J Dent 2021; 15 (01) 33-38
    Article in Thieme ConnectPubMedGoogle Scholar
  • 25 Huang MH, Chang LW, Sung WC, Vong WJ, Wang BS. Protective effects of three smoke flavouring phenols on oxidative damage and nitric oxide production. Food Chem 2011; 126 (04) 1655-1661
    CrossrefPubMedGoogle Scholar
  • 26 Surboyo MDC, Ernawati DS, Radithia D. et al. Distilled liquid smoke coconut shell attenuates the cytokine profile of macrophages in oral ulcer in experimental model of diabetes mellitus. J Appl Pharm Sci 2021; 11 (08) 62-69
    Google Scholar
  • 27 Rodrigues S, da Costa AMR, Grenha A. Chitosan/carrageenan nanoparticles: effect of cross-linking with tripolyphosphate and charge ratios. Carbohydr Polym 2012; 89 (01) 282-289
    CrossrefPubMedGoogle Scholar
  • 28 Rodrigues S, Dionísio M, López CR, Grenha A. Biocompatibility of chitosan carriers with application in drug delivery. J Funct Biomater 2012; 3 (03) 615-641
    CrossrefPubMedGoogle Scholar
  • 29 Sahay G, Alakhova DY, Kabanov AV. Endocytosis of nanomedicines. J Control Release 2010; 145 (03) 182-195
    CrossrefPubMedGoogle Scholar
  • 30 Martien R, Adhyatmika A, Irianto IDK, Farida V, Sari DP. Perkembangan teknologi nanopartikel sebagai sistem penghantaran obat. Majalah Farmaseutik 2012; 8 (01) 133-144
    Google Scholar
  • 31 Amarasekara DS, Kim S, Rho J. Regulation of osteoblast differentiation by cytokine networks. Int J Mol Sci 2021; 22 (06) 1-16
    CrossrefPubMedGoogle Scholar