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DOI: 10.1055/s-0042-1743146
Changes in Migratory Speed Rate of Human Dental Pulp Stromal Cells Cultured in Advanced Platelet-Rich Fibrin
Abstract
Objective Migratory speed rate evaluation of human dental pulp stromal cells (hDP-SCs) is one of the important steps in dental pulp regeneration. Therefore, the aim of the study is to analyze various concentrations of advanced platelet-rich fibrin (A-PRF) culture media toward hDP-SCs' migratory speed rate evaluations.
Materials and Methods The hDP-SCs were divided into four groups: control: hDP-SCs in Dulbecco's modified Eagle medium + 10% fetal bovine serum group; hDP-SCs in 1% A-PRF group; hDP-SCs in 5% A-PRF group; and hDP-SCs in 10% A-PRF group, which were planted in 24-well (5 × 104 cell/well). The migratory speed rate of all groups was measured by using cell migration assay (scratch wound assay) after 24 hours. Cell characteristics were evaluated under microscope (Inverted microscope, Zeiss, Observer Z1, UK) that can be read through image-J interpretation. This image J represented the measurement of migratory speed rate (nm/h) data. Statistical analysis was conducted using one-way analysis of variance and post hoc Tamhane's test (p < 0.05) (IBM SPSS Statistics Software, version 22.0).
Results There was a statistically significant difference in the migratory speed rates of hDP-SCs among various concentration groups of A-PRF (1, 5, and 10%) compared with the control group.
Conclusion The increase in the migratory speed rate of hDP-SCs was highest in 10% A-PRF group.
Publication History
Article published online:
18 April 2022
© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
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References
- 1 Anh TMN, Ha LBT, Thuy AVP. In vitro evaluation of proliferation and migration behaviour of human bone marrow-derived mesenchymal stem cells in presence of platelet-rich plasma. Int J Dent 2019; 9639820
- 2 De Becker A, Riet IV. Homing and migration of mesenchymal stromal cells: how to improve the efficacy of cell therapy?. World J Stem Cells 2016; 8 (03) 73-87
- 3 Grada A, Otero-Vinas M, Prieto-Castrillo F, Obagi Z, Falanga V. Research techniques made simple: analysis of collective cell migration using the wound healing assay. J Invest Dermatol 2017; 137 (02) e11-e16
- 4 Pijuan J, Barceló C, Moreno DF. et al. In vitro cell migration, invasion, and adhesion assays: from cell imaging to data analysis. Front Cell Dev Biol 2019; 7: 107
- 5 Eramo S, Natali A, Pinna R, Milia E. Dental pulp regeneration via cell homing. Int Endod J 2018; 51 (04) 405-419
- 6 Iwasa SN, Babona-Pilipos R, Morshead CM. Environmental factors that influence stem cell migration: an “electric field”. Stem Cells Int 2017; 2017: 4276927
- 7 Rombouts C, Jeanneau C, Bakopoulou A, About I. Dental pulp stem cell recruitment signals within injured dental pulp tissue. Dent J (Basel) 2016; 4 (02) 8
- 8 Andreas K, Sittinger M, Ringe J. Toward in situ tissue engineering: chemokine-guided stem cell recruitment. Trends Biotechnol 2014; 32 (09) 483-492
- 9 Chmilewsky F, Jeanneau C, Dejou J, About I. Sources of dentin-pulp regeneration signals and their modulation by the local microenvironment. J Endod 2014; 40 (4, Suppl): S19-S25
- 10 Ray Jr HL, Marcelino J, Braga R, Horwat R, Lisien M, Khaliq S. Long-term follow up of revascularization using platelet-rich fibrin. Dent Traumatol 2016; 32 (01) 80-84
- 11 Bezgin T, Yilmaz AD, Celik BN, Kolsuz ME, Sonmez H. Efficacy of platelet-rich plasma as a scaffold in regenerative endodontic treatment. J Endod 2015; 41 (01) 36-44
- 12 Narang I, Mittal N, Mishra N. A comparative evaluation of the blood clot, platelet-rich plasma, and platelet-rich fibrin in regeneration of necrotic immature permanent teeth: a clinical study. Contemp Clin Dent 2015; 6 (01) 63-68
- 13 Naik B, Karunakar P, Jayadev M, Marshal VR. Role of platelet rich fibrin in wound healing: a critical review. J Conserv Dent 2013; 16 (04) 284-293
- 14 Strauss FJ, Nasirzade J, Kargarpoor Z, Stähli A, Gruber R. Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies. Clin Oral Investig 2020; 24 (02) 569-584
- 15 Maria PP, Amin S, Mayya A, Naik R. Platelet rich fibrin in regenerative endodontics: an update. Int J Appl Dent Sci. 2020; 6 (02) 25-29
- 16 Caymaz MG, Uyanik LO. Comparison of the effect of advanced platelet-rich fibrin and leukocyte- and platelet-rich fibrin on outcomes after removal of impacted mandibular third molar: a randomized split-mouth study. Niger J Clin Pract 2019; 22 (04) 546-552
- 17 Masuki H, Okudera T, Watanebe T. et al. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent 2016; 2 (01) 19
- 18 Chatterjee A, Debnath K. Comparative evaluation of growth factors from platelet concentrates: an in vitro study. J Indian Soc Periodontol 2019; 23 (04) 322-328
- 19 Ghanaati S, Booms P, Orlowska A. et al. Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol 2014; 40 (06) 679-689
- 20 Huang FM, Yang SF, Zhao JH, Chang YC. Platelet-rich fibrin increases proliferation and differentiation of human dental pulp cells. J Endod 2010; 36 (10) 1628-1632
- 21 Risya DM, Asrianti D, Margono A. The efficacy of platelet-rich fibrin lysate (PRF-L) for fibroblast cell proliferation. J Int Dent Med Res 2017; 10: 809-813
- 22 Saeed MA, El-Rahman MA, Helal ME, Zaher AR, Grawish ME. Efficacy of human platelet rich fibrin exudate vs fetal bovine serum on proliferation and differentiation of dental pulp stem cells. Int J Stem Cells 2017; 10 (01) 38-47
- 23 Illmilda AD, Margono A, Julianto I, Wardoyo MP. Advanced platelet rich fibrin (A-PRF) supplemented culture medium for human dental pulp stem cell proliferation. J Int Dent Med Res. 2019; 12 (02) 396-400
- 24 Bagio DA, Julianto I, Suprastiwi E, Margono A. Ideal concentration of advanced-platelet rich fibrin (A-PRF) conditioned media for human dental pulp stem cells differentiation. Pesqui Bras Odontopediatria Clin Integr 2019; 19 (01) 1-9
- 25 Fu X, Liu G, Halim A, Ju Y, Luo Q, Song AG. Mesenchymal stem cell migration and tissue repair. Cells 2019; 8 (08) 784
- 26 de Lucas B, Pérez LM, Gálvez BG. Importance and regulation of adult stem cell migration. J Cell Mol Med 2018; 22 (02) 746-754
- 27 Wen J, Li HT, Li SH, Li X, Duan JM. Investigation of modified platelet-rich plasma (mPRP) in promoting the proliferation and differentiation of dental pulp stem cells from deciduous teeth. Braz J Med Biol Res 2016; 49 (10) e5373
- 28 Piletz JE, Drivon J, Eisenga J. et al. Human cells grown with or without substitutes for fetal bovine serum. Cell Med 2018; 10 (10) 2155179018755140
- 29 Miron RJ, Zucchelli G, Pikos MA. et al. Use of platelet-rich fibrin in regenerative dentistry: a systematic review. Clin Oral Investig 2017; 21 (06) 1913-1927
- 30 Utami Dewi S, Margono A, Asrianti D, Fatmasari A. Effects of various concentration of lysate platelet-rich fibrin on human dental pulp stromal cell migration activity. Clin Oral Investig 2020; 24 (02) 569-584
- 31 Swastiningtyas S, Margono A, Asrianti D, Oktayani R, Yulianto I. Analysis of lysate platelet-rich fibrin effects on human dental pulp stem cell differentiation through dentin sialophosphoprotein expression. Int J App Pharm. 2020; 12 (02) 34-37
- 32 Glenn HL, Messner J, Meldrum DR. A simple non-perturbing cell migration assay insensitive to proliferation effects. Sci Rep 2016; 6: 31694
- 33 Akpinar G, Kasap M, Aksoy A, Duruksu G, Gacar G, Karaoz E. Phenotypic and proteomic characteristics of human dental pulp derived mesenchymal stem cells from a natal, an exfoliated deciduous, and an impacted third molar tooth. Stem Cells Int 2014; 2014: 457059
- 34 Yang JW, Zhang YF, Wan CY. et al. Autophagy in SDF-1α-mediated DPSC migration and pulp regeneration. Biomaterials 2015; 44: 11-23
- 35 Pan S, Dangaria S, Gopinathan G. et al. SCF promotes dental pulp progenitor migration, neovascularization, and collagen remodeling - potential applications as a homing factor in dental pulp regeneration. Stem Cell Rev Rep 2013; 9 (05) 655-667
- 36 Caruana A, Savina D, Macedo JP, Soares SC. From platelet-rich plasma to advanced platelet-rich fibrin: biological achievements and clinical advances in modern surgery. Eur J Dent 2019; 13 (02) 280-286