Planta Med 2017; 83(08): 684-692
DOI: 10.1055/s-0042-121754
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Antiobesity and Lipid Lowering Effects of Orthosiphon stamineus in High-Fat Diet-Induced Obese Mice

Atefehalsadat Seyedan
1  Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
,
Mohammed Abdullah Alshawsh
1  Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
,
Mustafa Ahmed Alshagga
1  Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
2  School of Biomedical Sciences, Faculty of Science, University of Nottingham Malaysia Campus, Semenyih, Selangor, Malaysia
,
Zahurin Mohamed
1  Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
› Author Affiliations
Further Information

Publication History

received 01 August 2016
revised 01 November 2016

accepted 14 November 2016

Publication Date:
19 December 2016 (online)

Abstract

The present study investigated the antiobesity and lipid lowering effects of an ethanolic extract of leaves obtained from Orthosiphon stamineus (200 and 400 mg/kg) and its major compound (rosmarinic acid, 10 mg/kg) in obese mice (C57BL/6) induced by a high-fat diet. Continuous supplementation with O. stamineus extract (200 and 400 mg/kg) for 8 weeks significantly decreased body weight gain (p < 0.05). However, supplementation with rosmarinic acid, a constituent in the extract, produced only a slight reduction in body weight gain compared to the high-fat diet control group. Food intake between the treatment and the high-fat diet groups was similar, which suggested that the plant extract did not suppress food intake. Further, body weight reduction of the treatment groups was not due to a decreased reduction in energy intake. Compared to the high-fat diet-fed group, serum triglycerides, total cholesterol, and low-density lipoprotein cholesterol levels were significantly reduced in the treated groups, while high-density lipoprotein cholesterol levels were not significantly altered. Accumulation of hepatic lipid droplets induced by a high-fat diet was markedly inhibited by O. stamineus extract. In addition, O. stamineus significantly diminished liver malondialdehyde production, and significantly elevated the activities of hepatic superoxidase dismutase. The present study showed that an ethanolic extract prepared from the leaves of O. stamineus can significantly reduce a gain in body weight, enhance antioxidant activity, and possess hypolipidemic and antiobesity effects, thereby protecting against the adverse effects of high-fat diet-induced obesity.

Supporting Information

Determination of the phytochemical contents (total saponins, flavonoids, and phenolics) of the O. stamineus extract is available as Supporting Information (Table 1S). The composition of the HFD (TD.06 414, 60 % fat) (Table 2S) and an HPLC/UV chromatogram of rosmarinic acid in O. stamineus (Fig. 1S) are also available as Supporting Information.