Int J Sports Med 2017; 38(06): 473-480
DOI: 10.1055/s-0042-121638
Immunology
© Georg Thieme Verlag KG Stuttgart · New York

Aerobic Exercise Decreases Lung Inflammation by IgE Decrement in an OVA Mice Model

Deborah Camargo Hizume-Kunzler
1  State University of Santa Catarina, Physical Therapy, Florianopolis, Brazil
,
Flavia R. Greiffo
2  Nove de Julho University – UNINOVE, Research, São Paulo, Brazil
,
Bárbara Fortkamp
1  State University of Santa Catarina, Physical Therapy, Florianopolis, Brazil
,
Gabriel Ribeiro Freitas
3  Universidade do Estado de Santa Catarina Centro de Ciencias da Departamento de Fisioterapia, Saude e do Esporte, Florianopolis, Brazil
,
Juliana Keller Nascimento
3  Universidade do Estado de Santa Catarina Centro de Ciencias da Departamento de Fisioterapia, Saude e do Esporte, Florianopolis, Brazil
,
Thayse Regina Bruggemann
4  School of Medicine of University of Sao Paulo, Internal Medicine, Sao Paulo, Brazil
,
Leonardo Melo Avila
1  State University of Santa Catarina, Physical Therapy, Florianopolis, Brazil
,
Adenir Perini
5  Faculdade de Medicina da Universidade de São Paulo, Departamento de Clínica Médica, São Paulo, Brazil
,
Franciane Bobinski
6  Department of Biological Science, Federal University of Santa Catarina, Florianopolis, Brazil
,
Morgana Duarte Silva
6  Department of Biological Science, Federal University of Santa Catarina, Florianopolis, Brazil
,
Fernanda Rocha Lapa
7  Universidade do Contestado Campus Concordia, Ciências Biológicas, Concordia, Brazil
,
Rodolfo Paula Vieira
8  Nove de Julho University (UNINOVE), Laboratory of Pulmonary and Exercise Immunology (LABPEI) and Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), Sao Paulo, Brazil
,
Verônica Vargas Horewicz
9  Universidade Federal de Santa Catarina, Departamento de Farmacologia, Florianopolis, Brazil
,
Adair Roberto Soares dos Santos
10  Universidade Federal de Santa Catarina, Departamento de Ciências Biológicas, Florianópolis, Brazil
,
Kelly Cattelan Bonorino
10  Universidade Federal de Santa Catarina, Departamento de Ciências Biológicas, Florianópolis, Brazil
› Author Affiliations
Further Information

Publication History



accepted after revision 25 October 2016

Publication Date:
07 April 2017 (online)

Abstract

Aerobic exercise (AE) reduces lung function decline and risk of exacerbations in asthmatic patients. However, the inflammatory lung response involved in exercise during the sensitization remains unclear. Therefore, we evaluated the effects of exercise for 2 weeks in an experimental model of sensitization and single ovalbumin-challenge. Mice were divided into 4 groups: mice non-sensitized and not submitted to exercise (Sedentary, n=10); mice non-sensitized and submitted to exercise (Exercise, n=10); mice sensitized and exposed to ovalbumin (OVA, n=10); and mice sensitized, submitted to exercise and exposed to OVA (OVA+Exercise, n=10). 24 h after the OVA/saline exposure, we counted inflammatory cells from bronchoalveolar fluid (BALF), lung levels of total IgE, IL-4, IL-5, IL-10 and IL-1ra, measurements of OVA-specific IgG1 and IgE, and VEGF and NOS-2 expression via western blotting. AE reduced cell counts from BALF in the OVA group (p<0.05), total IgE, IL-4 and IL-5 lung levels and OVA-specific IgE and IgG1 titers (p<0.05). There was an increase of NOS-2 expression, IL-10 and IL-1ra lung levels in the OVA groups (p<0.05). Our results showed that AE attenuated the acute lung inflammation, suggesting immunomodulatory properties on the sensitization process in the early phases of antigen presentation in asthma.