PMIO 2017; 4(01): e1-e7
DOI: 10.1055/s-0042-121608
Georg Thieme Verlag KG Stuttgart · New York

Inhibition of Degranulation of RBL-2H3 Cells by Extracts and Compounds from Armillaria ostoyae

Simon Merdivan1, Kristina Jenett-Siems2, Karsten Siems3, Timo Niedermeyer4, Michael J. Solis5, 6, Martin Unterseher5, Ulrike Lindequist1
  • 1Institute of Pharmacy, Ernst-Moritz-Arndt University Greifswald, Greifswald, Germany
  • 2Institute of Pharmacy, Freie Universität Berlin, Berlin, Germany
  • 3AnalytiCon Discovery GmbH, Potsdam, Germany
  • 4Interfaculty Institute of Microbiology and Infection Medicine, Eberhard Karls University Tübingen, Tübingen, Germany
  • 5Institute of Botany and Landscape Ecology, Ernst-Moritz-Arndt University Greifswald, Greifswald, Germany
  • 6Department of Natural Sciences, College of Science and Information Technology, Ateneo de Zamboanga University, Zamboanga City, Philippines
Further Information

Publication History

received 22 July 2016
revised 04 November 2016

accepted 09 November 2016

Publication Date:
16 February 2017 (online)


Armillaria ostoyae (Romagn.) Herink is an edible honey mushroom from the family Physalacriaceae (Agaricales, Basidiomycota). Dichloromethane extracts of mushroom mycelium and fruiting bodies exhibited a significant degranulation inhibiting effect on RBL-2H3 cells using noncytotoxic concentrations. Bioactivity-guided fractionation of the mycelial dichloromethane extract led to the isolation of sesquiterpen aryl esters. Methyl linoleate could also be isolated. These substances were obtained from A. ostoyae for the first time, with one compound representing an undescribed natural product. Purified compounds melleolide H and J inhibited degranulation significantly. A. ostoyae could be a candidate for support of allergy treatments.

Supporting Information

Graphics from RAxML and MrBayes for species determination of the mycelium of A. ostoyae (Fig. S1S2), CD, ATIR, and UV spectra and 1H- as well as 1H,1H-COSY NMR spectra of 1 (Fig. S3S8), and LC chromatograms of crude extracts and active fractions (Fig. S9S11) are available as Supporting Information.