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DOI: 10.1055/s-0042-121608
Inhibition of Degranulation of RBL-2H3 Cells by Extracts and Compounds from Armillaria ostoyae
Publication History
received 22 July 2016
revised 04 November 2016
accepted 09 November 2016
Publication Date:
16 February 2017 (online)
Abstract
Armillaria ostoyae (Romagn.) Herink is an edible honey mushroom from the family Physalacriaceae (Agaricales, Basidiomycota). Dichloromethane extracts of mushroom mycelium and fruiting bodies exhibited a significant degranulation inhibiting effect on RBL-2H3 cells using noncytotoxic concentrations. Bioactivity-guided fractionation of the mycelial dichloromethane extract led to the isolation of sesquiterpen aryl esters. Methyl linoleate could also be isolated. These substances were obtained from A. ostoyae for the first time, with one compound representing an undescribed natural product. Purified compounds melleolide H and J inhibited degranulation significantly. A. ostoyae could be a candidate for support of allergy treatments.
Key words
Armillaria ostoyae - Physalacriaceae - RBL-2H3 - sesquiterpene aryl esters - β-hexosaminidase assay - melleolide - cytotoxicitySupporting Information
Graphics from RAxML and MrBayes for species determination of the mycelium of A. ostoyae (Fig. S1–S2), CD, ATIR, and UV spectra and 1H- as well as 1H,1H-COSY NMR spectra of 1 (Fig. S3–S8), and LC chromatograms of crude extracts and active fractions (Fig. S9–S11) are available as Supporting Information.
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