Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine model

Shinichiro Kobayashi; Nobuo Kanai; Nobuyuki Tanaka; Masanori Maeda; Takahiro Hosoi; Fumio Fukai; Susumu Eguchi; Masayuki Yamato

doi:10.1055/s-0042-116145

Endoscopy International Open, Table of Contents

Endoscopy 2016; 04(11): E1116-E1123
DOI: 10.1055/s-0042-116145

Original article

Transplantation of epidermal cell sheets by endoscopic balloon dilatation to avoid esophageal re-strictures: initial experience in a porcine model

Shinichiro Kobayashi

¹Department of Surgery, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan

²Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan

,

Nobuo Kanai

²Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan

,

Nobuyuki Tanaka

²Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan

³Laboratory for Integrated Biodevice, Quantitative Biology Center – RIKEN, Fukita, Japan

,

Masanori Maeda

²Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan

,

Takahiro Hosoi

²Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan

⁴Department of Pharmaceutical Sciences, Graduate School of Pharmaceutical Science, Tokyo University of Science, Noda, Japan

,

Fumio Fukai

⁴Department of Pharmaceutical Sciences, Graduate School of Pharmaceutical Science, Tokyo University of Science, Noda, Japan

,

Susumu Eguchi

¹Department of Surgery, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan

,

Masayuki Yamato

²Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan

› Author Affiliations

Abstract

Background and study aims: Epidermal cell sheet (ECS) transplantation immediately after aggressive endoscopic submucosal dissection (ESD) has been shown to be safe and effective in the prevention of esophageal strictures. This study evaluated the feasibility of ECS transplantation after endoscopic balloon dilation (EBD) in a porcine model.

Methods: Six pigs underwent circumferential esophageal ESD under general anesthesia. Two weeks later, two pigs underwent EBD and transplantation of an autologous ECS, two underwent EBD alone, and two underwent endoscopic observation only (control).

Results: The two pigs in the transplantation group underwent six ECS transplants after EBD with five of the six (83 %) being successful, as shown by engraftment of transplanted ECSs after 7 days. No adverse events were observed. Stricture rates were lower in the two transplanted pigs (55 % and 60 %) than in the control (92.2 % and 87.7 %) and EBD-treated (71.7 % and 78.2 %) pigs. Infiltration of inflammatory cells was significantly lower in the transplanted pigs than in the control and EBD-treated pigs.

Conclusion: Preliminary results indicate the stability of the ECS transplantation procedure and the engraftment of transplanted ECS in the tears after EBD. This proof-of-concept study suggests that covering tears with ECSs after EBD may avoid re-strictures.

Full Text

References

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