Endosc Int Open 2016; 04(10): E1090-E1095
DOI: 10.1055/s-0042-115173
Original article
© Georg Thieme Verlag KG Stuttgart · New York

Application of a silver coating on plastic biliary stents to prevent biofilm formation: an experimental study using electron microscopy

Akane Yamabe
1  Gastroenterology, Aizu Medical Center, Fukushima Medical University, Aizuwakamatsu, Japan
,
Atsushi Irisawa
1  Gastroenterology, Aizu Medical Center, Fukushima Medical University, Aizuwakamatsu, Japan
,
Ikuo Wada
2  Department of Cell Science, Institute of Biomedical Science, Fukushima Medical University School of Medicine, Fukushima, Japan
,
Goro Shibukawa
1  Gastroenterology, Aizu Medical Center, Fukushima Medical University, Aizuwakamatsu, Japan
,
Mariko Fujisawa
1  Gastroenterology, Aizu Medical Center, Fukushima Medical University, Aizuwakamatsu, Japan
,
Ai Sato
1  Gastroenterology, Aizu Medical Center, Fukushima Medical University, Aizuwakamatsu, Japan
,
Ryo Igarashi
1  Gastroenterology, Aizu Medical Center, Fukushima Medical University, Aizuwakamatsu, Japan
,
Takumi Maki
1  Gastroenterology, Aizu Medical Center, Fukushima Medical University, Aizuwakamatsu, Japan
,
Koki Hoshi
1  Gastroenterology, Aizu Medical Center, Fukushima Medical University, Aizuwakamatsu, Japan
› Author Affiliations
Further Information

Publication History

submitted21 March 2016

accepted after revision29 July 2016

Publication Date:
14 September 2016 (eFirst)

Background and study aims: Biliary stent dysfunction is mainly caused by biliary sludge that forms as a result of bacterial adherence and subsequent biofilm formation on the inner surface of the stent. Silver ions arewell known to have excellent antimicrobial activity against a wide range of microorganisms. In this study, we designed and constructed silver-coated plastic stent (PS) and investigated whether the silver coating prevented bacterial adherence and biofilm formation through the use of electron microscopy.

Material and methods: The polyurethane PS with/without silver coating were prepared in 6-inch segments. The silver-based antimicrobial agents were electrostatically applied onto the stent surface. The stents were then immersed for 5 weeks in infected human bile juice obtained from a patient with cholangitis, and electron microscopy was used to investigate the ability of the modified PS to prevent bacterial adherence and biofilm formation.

Results: The bacterial flora did not change before and after immersion of stents in both the group with and without silver coating. Electron microscopic observation revealed meshwork-like structures around the bacteria, characteristic of biofilm-forming bacteria, in all stents from the control group (6/6, 100 %). On the other hand, a limited number of bacteria were observed in all stents in the silver-coated group, and no apparent biofilm formation was observed (0/6, 0 %).

Conclusions: The significance of the findings from our study is the ability of silver-coated PS to prevent biofilm formation on the stent surface, which results in the prevention of stent occlusion.