Cytotoxic New Nortriterpene from Roots of Potentilla atrosanguinea var. argyrophylla and its UPLC Quantification

Abstract

Potentilla atrosanguinea is well known for its ethnomedicinal uses since ancient times. The present study includes the isolation of a new nortriterpene, 28-methyl-acanthochlamate (1), from the roots of P. atrosanguinea. The structure of 1 was established from HR-ESI-MS and NMR spectroscopic analysis. Its relative stereochemistry was set with the help of NOESY correlation experiment. Compound (1) has been quantified by a new developed UPLC-DAD method (1.1 mg/g). The in vitro cytotoxicity of the new compound (1) was evaluated by sulforhodamine B assay against three cancer cells; human cervical cancer (SiHa), epidermal carcinoma (KB), and human adenocarcinoma (Colo-205). The new isolated compound (1) showed a significantly higher cytotoxicity against all the cells (SiHa, IC₅₀ 30.5 µg/mL; KB, IC₅₀ 22.6 µg/mL and Colo-205, IC₅₀ 18.8 µg/mL).

Key words

Potentilla atrosanguinea - Rosaceae - 28-methyl-acanthochlamate - quantification - cytotoxicity

• References

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