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Planta Med 2016; 82(13): 1173-1179
DOI: 10.1055/s-0042-108209
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

A Hydroalcoholic Extract from Paullinia pinnata L. Roots Exerts Anthelmintic Activity against Free-Living and Parasitic Nematodes

Verena Spiegler
1   Institute for Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany
,
Eva Liebau
2   Department of Molecular Physiology, Institute for Zoophysiology, Münster, Germany
,
Carolin Peppler
1   Institute for Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany
,
Katharina Raue
3   Institute for Parasitology, University of Veterinary Medicine Hannover, Hannover, Germany
,
Steffen Werne
4   Research Institute of Organic Agriculture (FiBL), Frick, Switzerland
,
Christina Strube
3   Institute for Parasitology, University of Veterinary Medicine Hannover, Hannover, Germany
,
Felix Heckendorn
4   Research Institute of Organic Agriculture (FiBL), Frick, Switzerland
,
Christian Agyare
5   Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
,
Timo Stark
6   Food Chemistry & Molecular Sensory Science, Technical University of Munich, Freising, Germany
,
Thomas Hofmann
6   Food Chemistry & Molecular Sensory Science, Technical University of Munich, Freising, Germany
,
Andreas Hensel
1   Institute for Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany
› Author Affiliations
Further Information

Publication History

received 15 March 2016
revised 22 April 2016

accepted 30 April 2016

Publication Date:
10 June 2016 (online)

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Abstract

Paullinia pinnata is a medicinal plant traditionally used in West Africa against a wide range of diseases including soil-transmitted helminthiases. In this study, a hydroethanolic root extract was investigated for its phytochemical composition and in vitro activity against the free-living nematode Caenorhabditis elegans as well as the larval stages of the parasitic helminths Ancylostoma caninum, Haemonchus contortus, Toxocara cati, and Trichuris vulpis.

LC-MS analysis of the ethanol-water (1 : 1) extract revealed epicatechin and different A-type linked oligomeric and polymeric procyanidins as the predominant compounds.

Within an in vitro mortality assay, the extract showed a lethal activity against T. cati (LC50 of 112 µg/mL), T. vulpis (LC50 of 17 µg/mL), and C. elegans (LC50 2.5 of mg/mL), but not against A. caninum. Additionally, effects on egg hatching and larval migration of H. contortus were investigated, but no inhibitory activity was observed.

Overall, these findings rationalize the traditional use of the root extract from P. pinnata as an anthelmintic remedy and provide insight into the phytochemical composition of the extract.

Key words

Paullinia pinnata - Sapindaceae - A-type procyanidin - Caenorhabditis elegans - Toxocara cati - Trichuris vulpis - Ancylostoma caninum - Haemonchus contortus

Supporting Information

 

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