Int J Sports Med 2016; 37(11): 841-848
DOI: 10.1055/s-0042-107247
Physiology & Biochemistry
© Georg Thieme Verlag KG Stuttgart · New York

Resistance Training and Ovariectomy: Antagonic Effects in Mitochondrial Biogenesis Markers in Rat Skeletal Muscle

M. R. Barbosa
1  Education in Health, Universidade Federal de Sergipe, Lagarto, Brazil
,
G. E. Shiguemoto
2  Department of Physiological Sciences, Federal University of São Carlos, São Carlos, Brazil
,
L. M. Tomaz
3  Physiological Sciences, Federal University of Sao Carlos, Ribeirão Preto, Brazil
,
F. C. Ferreira
4  Physiological Sciences, Federal University of Sao Carlos, São Carlos, Brazil
,
M. F. C. Rodrigues
5  Ciências Fisiológicas, Universidade Federal de São Carlos, São Carlos, Brazil
,
M. M. Domingues
5  Ciências Fisiológicas, Universidade Federal de São Carlos, São Carlos, Brazil
,
M. V. C. Souza Master
6  Department of Physiological Sciences, Laboratory of Exercise Physiology, Federal University of São Carlos, São Carlos, Brazil
,
G. H. R. Canevazzi
3  Physiological Sciences, Federal University of Sao Carlos, Ribeirão Preto, Brazil
,
N. S. Silva-Magosso
7  Physiological Sciences – Physiology of Exercise, Federal University of São Carlos, São Carlos, Brazil
,
H. S. Selistre-de-Araujo
5  Ciências Fisiológicas, Universidade Federal de São Carlos, São Carlos, Brazil
,
S. EA Perez
8  Department of Phsysiological Sciences, Federal University of São Carlos (UFSCar), São Carlos, Brazil
› Author Affiliations
Further Information

Publication History



accepted after revision 12 April 2016

Publication Date:
18 July 2016 (online)

Abstract

Estrogen reduction is associated with a decline in skeletal muscle mitochondrial biogenesis. Molecular events associated with improvements in markers of mitochondrial biogenesis after resistance training and estradiol replacement are unknown. This study aimed to investigate the effects of ovariectomy, resistance training, and estradiol replacement on markers of mitochondrial biogenesis and protein expression related to oxidative capacity in the rat gastrocnemius pool. Estradiol replacement was performed using Silastic® capsules. During the 12-week resistance training, animals climbed a ladder with weights attached to their tails. Gene expression was analysed by RT-PCR, and protein content was determined by western blotting. Ovariectomy decreased the gene expression of the mitochondrial biogenesis markers PGC-1α (~73%), NRF-1 (~44%), and TFAM (~53%) (p<0.05) and decreased the protein expression of phosphorylated AMPK, CREB and AKT, which are related to oxidative capacity. Resistance training increased PGC-1α (~59%) and TFAM (~48%) expression compared to the Ovariectomy-Sedentary group. The combination of resistance training and estradiol replacement was superior to the ovariectomy-sedentary and ovariectomy-resistance training treatments regarding the gastrocnemius muscle. Estrogen deficiency altered the expression of genes and proteins that favour the development of a mitochondrial dysfunction phenotype, which was improved with resistance training and was partially improved by estradiol replacement.