Subscribe to RSS
DOI: 10.1055/s-0042-106170
LC-MS-Based Quality Assessment of a Traditional Chinese Medicine YANG XIN Formulation
Publication History
received 18 January 2016
revised 21 March 2016
accepted 28 March 2016
Publication Date:
28 April 2016 (online)
Abstract
YANG XIN is a traditional Chinese medicine formulation used for nervous fatigue and consists of a proprietary blend of concentrated extracts from 18 plant ingredients. The 18 constituent plant ingredients, YANG XIN capsules, and formulations 2014–005_1 A and 1B were extracted by consecutive 24-hour macerations with dichloromethane followed by methanol. Metabolite separation was carried out through LC-MS in 40 minutes. Data acquisitions for qualitative and quantitative analyses of the samples were collected under (±) ESI modes and (+) APCI mode using full spectrum scan analysis.
A total of 18 analytical markers were identified by LC-MS for YANG XIN formulations based on accurate mass measurements, molecular formula, double bond equivalent, MFG score, and error (ppm) of the measurement. Aditionally, a comparison of the data with previously reported results for the compounds, followed by identity confirmation with standard compounds, was performed. Seventeen analytical markers representing 17 plant ingredients in the different YANG XIN formulations were quantified for the first time. The YANG XIN capsules and the 2014–005_1B formulation were similar to each other and different from the 2014–005_1 A formulation based on the fact that both YANG XIN capsules and the 2014–005_1B formulation contain the same analytical markers. This method provides good linearity (r2 > 0.9945), intraday precision (R. S. D. < 3.9 %), interday precision (R. S. D. < 5.6 %), accuracy (99.2–101 %), recovery (145.7 %), limit of detection (0.0011–0.0732 µg/mL), and limit of quantitation (0.0038–0.2441 µg/mL).
-
References
- 1 Xia YG, Yang BY, Liang J, Yang Q, Wang D, Kuang HX. Quantitative analysis and fingerprint profiles for quality control of Fructus Schisandrae by gas chromatography: mass spectrometry. Sci World J 2014; 2014: 806759
- 2 Chen L, Qi J, Chang Y, Zhu D, Yu B. Identification and determination of the major constituents in traditional Chinese medicinal formula Danggui-Shaoyao-San by HPLC-DAD-ESI-MS/MS. J Pharm Biomed Anal 2009; 50: 127-137
- 3 Liang X, Zhang X, Dai W, Lv Y, Yan S, Zhang W. A combined HPLC-PDA and HPLC-MS method for quantitative and qualitative analysis of 10 major constituents in the traditional Chinese medicine Zuo Gui Wan. J Pharm Biomed Anal 2009; 49: 931-936
- 4 Yang D, An Y, Jiang X, Tang D, Gao Y, Zhao H, Wu X. Development of a novel method combining HPLC fingerprint and multi-ingredients quantitative analysis for quality evaluation of traditional Chinese medicine preparation. Talanta 2011; 85: 885-890
- 5 Chen R, Moriya J, Yamakawa J, Takahashi T, Kanda T. Traditional Chinese medicine for chronic fatigue syndrome. Evid Based Complement Alternat Med 2010; 7: 3-10
- 6 Chen H, Shi Y, Wei M, Liu W, Feng F. Chemical profile of the active fraction of Yi-Gan San by HPLC-DAD-Q-TOF-MS and its neuroprotective effect against glutamate-induced cytotoxicity. Chin J Nat Med 2014; 12: 869-880
- 7 Gai Y, Chen H, Wu C, Feng F, Wang Y, Liu W, Wang S. Analysis of the traditional medicine YiGan San by the fragmentation patterns of cadambine indole alkaloids using HPLC coupled with high-resolution MS. J Sep Sci 2013; 36: 3723-3732
- 8 Health Canada. Quality of Natural Health Products Guide. Technical tips online. Available at. http://www.hc-sc.gc.ca/dhp-mps/prodnatur/legislation/docs/eq-paq-eng.php%23a21319 Accessed December 17, 2015
- 9 Ryu M, Kim E, Chun M, Kang S, Shim B, Yu YB, Jeong G, Lee JS. Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p 38 and Erk. J Ethnopharmacol 2008; 115: 184-193
- 10 Löffler C, Czygan FC, Proksch P. Phenolic constituents as taxonomic markers in the genus Cuscuta (Cuscutaceae). Biochem Syst Ecol 1997; 25: 297-303
- 11 Zhang F, Li X, Li Z, Xu X, Peng B, Qin X, Du G. UPLC/Q-TOF MS-based metabolomics and qRT-PCR in enzyme gene screening with key role in triterpenoid saponin biosynthesis of Polygala tenuifolia . PLoS One 2014; 9: e105765
- 12 Liu J, Chen X, Yang W, Zhang S, Wang F, Tang Z. Chemical fingerprinting of Wild Germplasm Resource of Ophiopogon japonicus from Sichuan Basin, China by RP-HPLC coupled with hierarchical cluster analysis. Anal Lett 2010; 43: 2411-2423
- 13 Wagner H, Bauer R, Melchart D, Xiao PG, Staudinger A. Chromatographic fingerprint analysis of herbal medicines: thin-layer and high performance liquid chromatography of Chinese drugs. Vienna: Springer; 2011: 37-44
- 14 Xia B, Zhou Y, Tan HS, Ding L, Xu XH. Advanced ultra-performance liquid chromatography–photodiode array–quadrupole time-of-flight mass spectrometric methods for simultaneous screening and quantification of triterpenoids in Poria cocos . Food Chem 2014; 152: 237-244
- 15 Jiang Y, Tu PF. Analysis of chemical constituents in Cistanche species. J Chromatogr A 2009; 1216: 1970-1979