Download PDF
Planta Medica Letters 2016; 3(04): e87-e90
DOI: 10.1055/s-0042-105296
Letter
Georg Thieme Verlag KG Stuttgart · New York

In Vitro Rapid Multiplication Protocol for Ex Situ Conservation of the Rare, Endemic Medicinal Plant Achyranthes coynei

Mayuri Desai
1   Department of Pharmaceutical Biotechnology, KLE College of Pharmacy, Nehru Nagar, Belagavi, Karnataka State, India
,
Pramod J. Hurkadale
1   Department of Pharmaceutical Biotechnology, KLE College of Pharmacy, Nehru Nagar, Belagavi, Karnataka State, India
,
Vinayak Upadhya
2   Herbal Medicine Division, Regional Medical Research Centre, ICMR, Nehru Nagar, Belagavi, Karnataka State, India
,
Lalrinsangi Sailo
1   Department of Pharmaceutical Biotechnology, KLE College of Pharmacy, Nehru Nagar, Belagavi, Karnataka State, India
,
Harsha V. Hegde
2   Herbal Medicine Division, Regional Medical Research Centre, ICMR, Nehru Nagar, Belagavi, Karnataka State, India
3   Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, ICMR, Nehru Nagar, Belagavi, Karnataka State, India
,
Sandeep R. Pai
3   Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, ICMR, Nehru Nagar, Belagavi, Karnataka State, India
› Author Affiliations
Further Information

Publication History

received 30 December 2015
revised 26 February 2016

accepted 15 March 2016

Publication Date:
28 April 2016 (online)

   Permissions and Reprints

Abstract

Achyranthes coynei is a rare, endemic species with potential medicinal properties. The present study aimed to develop an efficient in vitro rapid multiplication protocol for A. coynei. It was successfully propagated in vitro using apical shoot tips as explants. A combination of 6-benzylaminopurine (3.0 mg · L−1), thidiazuron (0.5 mg · L−1), and kinetin (0.5 mg · L−1) in MS basal proved best for multiple shoot induction, yielding 4.8 shoots per explant after 60 days of culture. The shoots were rooted with a frequency of 80 % after 30 days of culture on MS agar medium fortified with IBA/NAA (0.5 mg · L−1) before being acclimatized in the greenhouse. The developed protocol will serve as a tool to restore the rare status of the plant and will append its sustainable utilization.

Key words

Achyranthes coynei - Amaranthaceae - in vitro - rapid multiplication - kinetin
 

  • References

  • 1 Pai SR, Upadhya V, Hegde HV, Kholkute SD. Achyranthes coynei Santapau, 1949 (Amranthaceae) – An addition to the flora of Karnataka, India. J Threat Taxa 2011; 3: 1875-1879
    CrossrefPubMedGoogle Scholar
  • 2 Mishra DK, Singh NP. Endemic and threatened flowering plants of Maharashtra. Calcutta: Botanical Survey of India, Ministry of Environment and Forests; 2001: 196-197
    Google Scholar
  • 3 Santapau HSJ. Flora of Khandala on Western Ghats of India. Records of the Botanical Survey of India, Vol. 16. Delhi: Botanical Survey of India; 1967: 224-225
    Google Scholar
  • 4 Santapau HSJ. Novitates Bombayenses. Kew Bull 1948; 3: 485-492
    CrossrefPubMedGoogle Scholar
  • 5 Singh NP, Raghavan RS. Endemic and threatened plants of western India. Ibid 1986; 8: 34
    PubMedGoogle Scholar
  • 6 Upadhya V, Ankad G, Pai SR, Hegde SV, Hegde HV. Preliminary pharmacognostic screening of Achyranthes coynei stem. J Ayurveda Integr Med 2015; 6: 134-138
    CrossrefPubMedGoogle Scholar
  • 7 Ankad G, Upadhya V, Pai SR, Hegde HV, Roy S. In vitro antimicrobial activity of Achyranthes coynei Sant. Asian Pac J Trop Dis 2013; 3: 930-935
    PubMedGoogle Scholar
  • 8 Ankad GM, Pai SR, Upadhya V, Hurkadale PJ, Hegde HV. Pharmacognostic evaluation of Achyranthes coynei leaf. Egypt J Basic Appl Sci 2015; 2: 25-31
    PubMedGoogle Scholar
  • 9 Upadhya V, Pai SR, Ankad G, Hurkadale PJ, Hegde HV. Phenolic contents and antioxidant properties from aerial parts of Achyranthes coynei Sant. Indian J Pharm Sci 2013; 75: 483-486
    CrossrefPubMedGoogle Scholar
  • 10 Upadhya V, Ankad GM, Pai SR, Hegde HV, Kholkute SD. Accumulation and trends in distribution of three triterpenoids from Achyranthes coynei determined using RP-UFLC analysis. Pharmacogn Mag 2014; 10: 398-401
    CrossrefPubMedGoogle Scholar
  • 11 Upadhya V, Ankad G, Pai SR, Hegde HV. Comparative HPTLC analysis of stem and leaf of Achyranthes coynei with Achyranthes aspera . Plant Sci Today 2015; 2: 7-10
    CrossrefPubMedGoogle Scholar
  • 12 Upadhya V, Hegde HV, Bhat S, Kholkute SD. Non-codified traditional medicine practices from Belgaum Region in Southern India: present scenario. J Ethnobiol Ethnomed 2014; 10: 49
    CrossrefPubMedGoogle Scholar
  • 13 Hegde HV, Upadhya V, Pai SR, Hegde GR, Hosur G, Mesta D. A preliminary inventory and management plan for medicinal plant conservation areas at Amgaon and Kakti in Belgaum district of Karnataka. Indian Forester 2013; 139: 843-850
    PubMedGoogle Scholar
  • 14 Mittermeier RA, Myers MN, Thomsen JB, da Fonesca GAB, Olivieri S. Biodiversity hotspots and major tropical wilderness areas: approaches to setting conservation priorities. Conserv Biol 1998; 12: 516-520
    CrossrefPubMedGoogle Scholar
  • 15 Martinez Pulido C, Harry IS, Torpe TA. Effect of various bud induction treatments on elongation and rooting of adventitious shoots of Canary Island pine (Pinus canariensis). Plant Cell Tissue Organ Cult 1994; 39: 225-230
    CrossrefPubMedGoogle Scholar
  • 16 Daffalla HH, Abdellatef E, Elhadi EA, Khalafalla MM. Effect of growth regulators on in vitro morphogenic response of Boscia senegalensis (Pers.) Lam. Poir. using mature zygotic embryos explants. Biotechnol Res Int 2011; 2011: 710758
    PubMedGoogle Scholar
  • 17 Annapurna D, Rathore TS. Direct adventitious shoot induction and plant regeneration of Embelia ribes Burm F. Plant Cell Tissue Organ Cult 2010; 101: 269-277
    CrossrefPubMedGoogle Scholar
  • 18 Srivastava K, Raghava N, Shagun. Raghava RP. Brassinosteroids stimulate seed germination parameters and chlorophyll content in Moongbean. Indian J Sci Res 2011; 2: 89-92
    PubMedGoogle Scholar